Journal article
NLRP3 Is a Critical Regulator of Inflammation and Innate Immune Cell Response during Mycoplasma pneumoniae Infection
Infection and immunity, Vol.86(1)
01/01/2018
Handle:
https://hdl.handle.net/2376/115232
PMCID: PMC5736809
PMID: 29061706
Abstract
Mycoplasma pneumoniae
is an atypical bacterial respiratory pathogen known to cause a range of airway inflammation and lung and extrapulmonary pathologies. We recently reported that an
M. pneumoniae
-derived ADP-ribosylating and vacuolating toxin called community-acquired respiratory distress syndrome (CARDS) toxin is capable of triggering NLRP3 (NLR-family, leucine-rich repeat protein 3) inflammasome activation and interleukin-1β (IL-1β) secretion in macrophages. However, it is unclear whether the NLRP3 inflammasome is important for the immune response during
M. pneumoniae
acute infection. In the current study, we utilized
in vitro
and
in vivo
models of
M. pneumoniae
infection to characterize the role of the NLRP3 inflammasome during acute infection.
M. pneumoniae-
infected macrophages deficient for inflammasome components NLRP3, ASC (apoptosis speck-like protein containing a caspase activation and recruitment domain), or caspase-1 failed to process and secrete IL-1β. The MyD88/NF-κB signaling pathway was found to be critical for proinflammatory gene expression in macrophages infected with
M. pneumoniae
. C57BL/6 mice deficient for NLRP3 expression were unable to produce IL-1β in the airways during acute infection, and lack of this inflammatory response led to deficient immune cell activation and delayed bacterial clearance. These findings are the first to report the importance of the NLRP3 inflammasome in regulating the inflammatory response and influencing the progression of
M. pneumoniae
during acute infection.
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Details
- Title
- NLRP3 Is a Critical Regulator of Inflammation and Innate Immune Cell Response during Mycoplasma pneumoniae Infection
- Creators
- Jesus A Segovia - Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USATe-Hung Chang - Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USAVicki T Winter - Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USAJacqueline J Coalson - Department of Pathology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USAMarianna P Cagle - Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USALavanya Pandranki - Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USASantanu Bose - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USAJoel B Baseman - Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USAThirumalai R Kannan - Department of Microbiology, Immunology and Molecular Genetics, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA
- Publication Details
- Infection and immunity, Vol.86(1)
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology; 1752 N St., N.W., Washington, DC
- Grant note
- K12GM111726 / ; U19AI070412 / ; R21AI118051; AI083387 / ; R21AI118051 / ;
- Identifiers
- 99900547804001842
- Language
- English
- Resource Type
- Journal article