Journal article
Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells
Biosensors & bioelectronics, Vol.26(2), pp.655-660
2010
Handle:
https://hdl.handle.net/2376/104263
PMID: 20673623
Abstract
Nanoscale enzyme reactors (NERs) of glucose oxidase in conductive mesoporous carbons were prepared in a two-step process of enzyme adsorption and follow-up enzyme crosslinking. MSU-F-C, a mesoprous carbon, has a bottleneck pore structure with mesocellular pores of 26
nm connected with window mesopores of 17
nm. This structure enables the ship-in-a-bottle mechanism of NERs, which effectively prevents the crosslinked enzymes in mesocellular pores from leaching through the smaller window mesopores. This NER approach not only stabilized the enzyme but also expedited electron transfer between the enzyme and the conductive MSU-F-C by maintaining a short distance between them. In a comparative study with GOx that was simply adsorbed without crosslinking, the NER approach was proven to be effective in improving the sensitivity of glucose biosensors and the power density of biofuel cells. The power density of biofuel cells could be further improved by manipulating several factors, such as by adding a mediator, changing the order of adsorption and crosslinking, and inserting a gold mesh as an electron collector.
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Details
- Title
- Nanoscale enzyme reactors in mesoporous carbon for improved performance and lifetime of biosensors and biofuel cells
- Creators
- Ki Young Kwon - Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon 305-701, South KoreaJongkyu Youn - Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon 305-701, South KoreaJae Hyun Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South KoreaYongjin Park - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South KoreaChulmin Jeon - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South KoreaByoung Chan Kim - Institut Pasteur Korea, Seongnam-si, Gyeonggi-do 463-400, South KoreaYongchai Kwon - Department of Chemical and Environmental Technology, Inha Technical College, Incheon 402-752, South KoreaXueyan Zhao - Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USAPing Wang - Bioproducts and Biosystems Engineering, Biotechnology Institute, University of Minnesota, St. Paul, MN 55108, USAByoung In Sang - Hazardous Substances Research Center, Korea Institute of Science and Technology, Seoul 136-791, South KoreaJinwoo Lee - Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology, Pohang, Gyungbuk 790-784, South KoreaHyun Gyu Park - Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon 305-701, South KoreaHo Nam Chang - Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon 305-701, South KoreaTaeghwan Hyeon - National Creative Research Initiative Center for Oxide Nanocrystalline Materials, School of Chemical Engineering, Seoul National University, Seoul 151-744, South KoreaSu Ha - Chemical Engineering Department, Washington State University, Pullman, WA 99164-2710, USAHee-Tae Jung - Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), Daejon 305-701, South KoreaJungbae Kim - Department of Chemical and Biological Engineering, Korea University, Seoul 136-701, South Korea
- Publication Details
- Biosensors & bioelectronics, Vol.26(2), pp.655-660
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546639401842
- Language
- English
- Resource Type
- Journal article