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Nanotips for single-step preparation of DNA for qPCR analysis
Journal article

Nanotips for single-step preparation of DNA for qPCR analysis

Dinesh Kalyanasundaram, Jong-Hoon Kim, Gareth Fotouhi, Hyun-Boo Lee, Morgan Hiraiwa, Kieseok Oh, Kyong-Hoon Lee and Jae-Hyun Chung
Analyst (London), Vol.138(11), pp.3135-3138
06/07/2013
DOI: https://doi.org/10.1039/c3an00170a
Handle:
https://hdl.handle.net/2376/105721
PMID: 23594970

Abstract

Nanotechnology - methods Humans Silicon Compounds - chemistry DNA, Viral - analysis Saliva - chemistry Carbon Compounds, Inorganic - chemistry Genome, Human - genetics DNA - blood Polymerase Chain Reaction - methods DNA - genetics DNA - isolation & purification Nanowires - chemistry Time Factors Analytic Sample Preparation Methods - methods DNA - analysis Bacteriophage lambda - genetics DNA, Viral - genetics
A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.

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