Journal article
Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens
ACS applied materials & interfaces, Vol.9(46), pp.40671-40680
11/22/2017
PMID: 28914522
Abstract
Nanozymes are an excellent class of optical reporters for the development of sensitive biosensors for widespread applications. In this study, mesoporous core-shell palladium@platinum (Pd@Pt) nanoparticles were synthesized and then applied as signal amplifier in a dual lateral flow immunoassay (LFIA) and integrated with a smartphone-based device for use in simultaneous detection of Salmonella Enteritidis and Escherichia coli O157:H7. After optimization, the limit of detections were calculated to be similar to 20 cfu/mL for S. Enteritidis and similar to 34 cfu/mL for E. coli O157:H7, respectively. The greatly improved sensitivity was contributed by the peroxidase-like catalytic activity of the Pd@Pt nanoparticles for signal enhancement and the parallel design of dual detection for eliminating the cross-interference. The estimated recoveries of the dual LFIA range from 91.44 to 117.00%, which indicated that the developed method is capable of detecting live bacteria in food samples. This approach provides an attractive platform for S. Enteritidis and E. coli 0157:H7 detection using a smartphone-based device as the sole piece of equipment, indicating great promise for foodborne pathogen analysis or infield food safety tracking.
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Details
- Title
- Nanozyme-Mediated Dual Immunoassay Integrated with Smartphone for Use in Simultaneous Detection of Pathogens
- Creators
- Nan Cheng - Washington State UniversityYang Song - Washington State UniversityMohamed M. A. Zeinhom - Beni-Suef UniversityYu-Chung Chang - Washington State UniversityLina Sheng - Washington State UniversityHaolin Li - China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Lab Food Qual & Safety, Beijing 100083, Peoples R ChinaDan Du - Washington State UniversityLei Li - Washington State UniversityMei-Jun Zhu - Washington State Univ, Sch Food Sci, Pullman, WA 99164 USAYunbo Luo - China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R ChinaWentao Xu - China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R ChinaYuehe Lin - Washington State Univ, Sch Mech & Mat Engn, Pullman, WA 99164 USA
- Publication Details
- ACS applied materials & interfaces, Vol.9(46), pp.40671-40680
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Amer Chemical Soc
- Number of pages
- 10
- Grant note
- WSU strartup fund 201606350119 / China Scholarship Council R01OH011023-01A1 / Centers for Disease Control and Prevention/National Institute for Occupational Safety and Health (CDC/NIOSH); United States Department of Health & Human Services; Centers for Disease Control & Prevention - USA; National Institute for Occupational Safety & Health (NIOSH)
- Identifiers
- 99901227637701842
- Language
- English
- Resource Type
- Journal article