Journal article
Natural Variation in Small Molecule–Induced TIR-NB-LRR Signaling Induces Root Growth Arrest via EDS1- and PAD4-Complexed R Protein VICTR in Arabidopsis[C][W]
The Plant cell, Vol.24(12), pp.5177-5192
12/2012
Handle:
https://hdl.handle.net/2376/113304
PMCID: PMC3556982
PMID: 23275581
Abstract
This chemical genetics work reveals natural variation in a newly identified R protein homolog, named VICTR, that produces primary root growth arrest in response to the small molecule DFPM. DFPM perception and signal transduction require early components of the plant
R
gene resistance signaling network, and the R protein VICTR coresides in complexes not only with EDS1 but also PAD4.
In a chemical genetics screen we identified the small-molecule [5-(3,4-dichlorophenyl)furan-2-yl]-piperidine-1-ylmethanethione (
DFPM
) that triggers rapid inhibition of early abscisic acid signal transduction via PHYTOALEXIN DEFICIENT4 (PAD4)- and ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1)-dependent immune signaling mechanisms. However, mechanisms upstream of EDS1 and PAD4 in
DFPM
-mediated signaling remain unknown. Here, we report that
DFPM
generates an
Arabidopsis thaliana
accession-specific root growth arrest in Columbia-0 (
Col-0
) plants. The genetic locus responsible for this natural variant,
VICTR
(
VARIATION IN COMPOUND TRIGGERED ROOT
growth response), encodes a
TIR
-
NB
-
LRR
(for Toll-Interleukin1 Receptor–nucleotide binding–Leucine-rich repeat) protein. Analyses of T-DNA insertion
victr
alleles showed that
VICTR
is necessary for
DFPM
-induced root growth arrest and inhibition of abscisic acid–induced stomatal closing. Transgenic expression of the
Col-0
VICTR
allele in
DFPM
-insensitive
Arabidopsis
accessions recapitulated the
DFPM
-induced root growth arrest.
EDS1
and
PAD4
, both central regulators of basal resistance and effector-triggered immunity, as well as
HSP90
chaperones and their cochaperones
RAR1
and
SGT1B
, are required for the
DFPM
-induced root growth arrest. Salicylic acid and jasmonic acid signaling pathway components are dispensable. We further demonstrate that VICTR associates with EDS1 and PAD4 in a nuclear protein complex. These findings show a previously unexplored association between a
TIR
-
NB
-
LRR
protein and PAD4 and identify functions of plant immune signaling components in the regulation of root meristematic zone-targeted growth arrest.
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Details
- Title
- Natural Variation in Small Molecule–Induced TIR-NB-LRR Signaling Induces Root Growth Arrest via EDS1- and PAD4-Complexed R Protein VICTR in Arabidopsis[C][W]
- Creators
- Tae-Houn Kim - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Hans-Henning Kunz - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Saikat Bhattacharjee - Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211-7310Felix Hauser - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Jiyoung Park - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Cawas Engineer - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Amy Liu - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Tracy Ha - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116Jane E Parker - Max-Planck Institute for Plant Breeding Research, Department of Plant-Microbe Interactions, D-50829 Cologne, GermanyWalter Gassmann - Division of Plant Sciences, Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, Missouri 65211-7310Julian I Schroeder - Division of Biological Sciences, Section of Cell and Developmental Biology, University of California at San Diego, La Jolla, California 92093-0116
- Publication Details
- The Plant cell, Vol.24(12), pp.5177-5192
- Academic Unit
- Medical Education and Clinical Science, Department of; Biological Sciences, School of
- Publisher
- American Society of Plant Biologists
- Identifiers
- 99900548168201842
- Language
- English
- Resource Type
- Journal article