Journal article
Neospora caninum-Infected Cattle Develop Parasite-Specific CD4+ Cytotoxic T Lymphocytes
Infection and immunity, Vol.71(6), pp.3272-3279
06/2003
Handle:
https://hdl.handle.net/2376/116170
PMCID: PMC155777
PMID: 12761108
Abstract
Cattle infected with
Neospora caninum
readily experience transplacental parasite transmission, presumably after maternal parasitemia, leading to abortion or birth of congenitally infected calves. Cytotoxic T lymphocytes (CTL) are important mediators of protective immunity against
Toxoplasma gondii
, an intracellular apicomplexan protozoan closely related to
N. caninum
. In this study,
N. caninum-
specific CTL expanded from peripheral blood mononuclear cells of two major histocompatibility complex-mismatched, experimentally infected cattle were identified by using a
51
Cr release cytotoxicity assay. Enrichment and blocking of CD4
+
- and CD8
+
-T-lymphocyte effector subsets indicated that CD4
+
CTL killed
N. caninum
-infected, autologous target cells and that killing was mediated through a perforin/granzyme pathway. Detection and characterization of CTL responses to
N. caninum
in the natural, outbred, bovine host will facilitate identification of immunogens and design of immunization strategies to induce parasite-specific CTL against transplacental
N. caninum
transmission in cattle.
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Details
- Title
- Neospora caninum-Infected Cattle Develop Parasite-Specific CD4+ Cytotoxic T Lymphocytes
- Creators
- Lauren M Staska - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Travis C McGuire - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Christopher J Davies - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Harris A Lewin - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164Timothy V Baszler - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164
- Publication Details
- Infection and immunity, Vol.71(6), pp.3272-3279
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547416301842
- Language
- English
- Resource Type
- Journal article