Journal article
Neuronal Microtubule-associated Protein 2D Is a Dual A-kinase Anchoring Protein Expressed in Rat Ovarian Granulosa Cells
The Journal of biological chemistry, Vol.279(26), pp.27621-27632
06/25/2004
Handle:
https://hdl.handle.net/2376/105436
PMCID: PMC1565147
PMID: 15056665
Abstract
A-kinase anchoring proteins (AKAPs) function to target protein kinase A (PKA) to specific locations within the cell. AKAPs are functionally identified by their ability to bind the type II regulatory subunits (RII) of PKA in an
in vitro
overlay assay. We previously showed that follicle-stimulating hormone (FSH) induces the expression of an 80-kDa AKAP (AKAP 80) in ovarian granulosa cells as they mature from a preantral to a preovulatory phenotype. In this report, we identify AKAP 80 as micro-tubule-associated protein 2D (MAP2D), a low molecular weight splice variant of the neuronal MAP2 protein. MAP2D is induced in granulosa cells by dexamethasone and by FSH in a time-dependent manner that mimics that of AKAP 80, and immunoprecipitation of MAP2D depletes extracts of AKAP 80. MAP2D is the only MAP2 protein present in ovaries and is localized to granulosa cells of preovulatory follicles and to luteal cells. MAP2D is concentrated at the Golgi apparatus along with RI and RII and, based on coimmunoprecipitation results, appears to bind both RI and RII in granulosa cells. Reduced expression of MAP2D resulting from treatment of granulosa cells with antisense oligonucleotides to MAP2 inhibited the phosphorylation of cAMP-response element-binding protein. These results suggest that this classic neuronal RII AKAP is a dual RI/RII AKAP that performs unique functions in ovarian granulosa cells that contribute to the preovulatory phenotype.
Metrics
8 Record Views
Details
- Title
- Neuronal Microtubule-associated Protein 2D Is a Dual A-kinase Anchoring Protein Expressed in Rat Ovarian Granulosa Cells
- Creators
- Lisa M Salvador - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611Maxfield P Flynn - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611Jesús Avila - Centro de Biologia Molecular “Severo Ochoa,” Facultad de Ciencias, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, SpainScott Reierstad - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611Evelyn T Maizels - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611Hena Alam - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611Youngkyu Park - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611John D Scott - Howard Hughes Medical Institute, Vollum Institute, Oregon Health and Science University, Portland, Oregon 97201-3098Daniel W Carr - Veterans Affairs Medical Center and Oregon Health and Science University, Portland, Oregon 97201-3098Mary Hunzicker-Dunn - From the Department of Cell and Molecular Biology, Northwestern University Feinberg School of Medicine, Chicago, Illinois 60611
- Publication Details
- The Journal of biological chemistry, Vol.279(26), pp.27621-27632
- Academic Unit
- Molecular Biosciences, School of
- Identifiers
- 99900547076501842
- Language
- English
- Resource Type
- Journal article