Journal article
New Latex Bead Agglutination Assay for Differential Diagnosis of Cattle Infected with Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis
Clinical and diagnostic laboratory immunology, Vol.11(6), pp.1070-1074
11/2004
Handle:
https://hdl.handle.net/2376/113832
PMCID: PMC524750
PMID: 15539508
Abstract
Extensive studies have shown that the current assays used to identify cattle infected with
Mycobacterium bovis
or
Mycobacterium avium
subsp.
paratuberculosis
are not sufficiently sensitive and specific to detect all infected animals, especially animals recently infected with the pathogens. In the present report we show that these limitations might be overcome with a latex bead agglutination assay (LBAA). With the specific immunodominant epitope (ESAT6-p) of
M. bovis
, we developed an LBAA and enzyme immunoassay (EIA) for that purpose and compared them with the “gold standard” culture method and skin test for their efficacy in detecting bovine tuberculosis. When sera from control healthy cows (
n
= 10),
M. avium
subsp.
paratuberculosis
-positive cattle (naturally infected,
n
= 16; experimentally infected,
n
= 8), and
M. bovis
-positive cattle (naturally infected,
n
= 49;experimentally infected,
n
= 20) were applied to an EIA and an LBAA developed with ESAT6-p, the two tests showed similar sensitivity (97.1% by EIA, 95.7% by LBAA), high specificity (94.2% by EIA, 100% by LBAA), and a positive correlation (kappa value, 0.85; correlation rate, 93.2%; correlation coefficient, 0.64). Receiver operating characteristic analysis of EIA results and comparison with the culture method determined a suitable cutoff value at 0.469, with an area under the curve of 0.991 (95% confidence interval, 0.977 to 1.0). As LBAA didn't show any positive reactions with sera from uninfected control cows or
M. avium
subsp
. paratuberculosis
-infected cattle, which were confirmed to be free of
M. bovis
by culture or PCR, LBAA using the ESAT6-p can be a rapid and useful
M. bovis
diagnostic assay. The data suggest that rapid, sensitive, and specific assays can be developed with peptides containing immunodominant epitopes present in proteins uniquely expressed in
M. bovis
or
M. avium
subsp
. paratuberculosis
for differential diagnosis of cattle infected with
M. bovis
or
M. avium
subsp
. paratuberculosis
.
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Details
- Title
- New Latex Bead Agglutination Assay for Differential Diagnosis of Cattle Infected with Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis
- Creators
- Hye Cheong Koo - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaYong Ho Park - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaJongsam Ahn - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaW. Ray Waters - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaMary Jo Hamilton - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaGeorge Barrington - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaAbdelaziz A Mosaad - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaMitch V Palmer - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaSang Shin - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South KoreaWilliam C Davis - Department of Microbiology, College of Veterinary Medicine, and School of Agricultural Biotechnology, Seoul National University, Seoul, South Korea
- Publication Details
- Clinical and diagnostic laboratory immunology, Vol.11(6), pp.1070-1074
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547989601842
- Language
- English
- Resource Type
- Journal article