Journal article
New generation NMR bioreactor coupled with high-resolution NMR spectroscopy leads to novel discoveries in Moorella thermoacetica metabolic profiles
Applied microbiology and biotechnology, Vol.98(19), pp.8367-8375
10/2014
Handle:
https://hdl.handle.net/2376/114782
PMID: 24946863
Abstract
An in situ nuclear magnetic resonance (NMR) bioreactor was developed and employed to monitor microbial metabolism under batch growth conditions in real time. We selected Moorella thermoacetica ATCC 49707 as a test case. M. thermoacetica (formerly Clostridium thermoaceticum) is a strictly anaerobic, thermophilic, acetogenic, gram-positive bacterium with potential for industrial production of chemicals. The metabolic profiles of M. thermoacetica were characterized during growth in batch mode on xylose (a component of lignocellulosic biomass) using the new generation NMR bioreactor in combination with high-resolution NMR (HR-NMR) spectroscopy. In situ NMR measurements were performed using water-suppressed H-1 NMR spectroscopy at 500 MHz, and aliquots of the bioreactor contents were taken for 600-MHz HR-NMR spectroscopy at specific intervals to confirm metabolite identifications and expand metabolite coverage. M. thermoacetica demonstrated the metabolic potential to produce formate, ethanol, and methanol from xylose, in addition to its known capability of producing acetic acid. Real-time monitoring of bioreactor conditions showed a temporary pH decrease, with a concomitant increase in formic acid during exponential growth. Fermentation experiments performed outside of the magnet showed that the strong magnetic field employed for NMR detection did not significantly affect cell metabolism. Use of the in situ NMR bioreactor facilitated monitoring of the fermentation process, enabling identification of intermediate and endpoint metabolites and their correlation with pH and biomass produced during culture growth. Real-time monitoring of culture metabolism using the NMR bioreactor in combination with HR-NMR spectroscopy will allow optimization of the metabolism of microorganisms producing valuable bioproducts.
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Details
- Title
- New generation NMR bioreactor coupled with high-resolution NMR spectroscopy leads to novel discoveries in Moorella thermoacetica metabolic profiles
- Creators
- Junfeng Xue - Bioproducts, Sciences and Engineering Laboratory Washington State University Tri-Cities 2710 Crimson Way Richland WA 99354-1671 USANancy Isern - Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99354 USAR Ewing - Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99354 USAAndrei Liyu - Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99354 USAJesse Sears - Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99354 USAHarlan Knapp - Eppendorf North America Hauppauge NY 11788 USAJens Iversen - Section for Sustainable Biotechnology Aalborg University 2450 Copenhagen DenmarkDaniel Sisk - Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory Richland WA 99354 USABirgitte Ahring - Bioproducts, Sciences and Engineering Laboratory Washington State University Tri-Cities 2710 Crimson Way Richland WA 99354-1671 USAPaul Majors - Bruker Biospin Corporation (formerly with the Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA 99354, USA) Billerica MA 01821 USA
- Publication Details
- Applied microbiology and biotechnology, Vol.98(19), pp.8367-8375
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Springer Berlin Heidelberg; Berlin/Heidelberg
- Identifiers
- 99900547556601842
- Language
- English
- Resource Type
- Journal article