New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

Sneha P Couvillion; Ying Zhu; Gabe Nagy; Joshua N Adkins; Charles Ansong; Ryan S Renslow; Paul D Piehowski; Yehia M Ibrahim; Ryan T Kelly; Thomas O Metz

doi:10.1039/c8an01574k

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New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

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New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells

Sneha P Couvillion, Ying Zhu, Gabe Nagy, Joshua N Adkins, Charles Ansong, Ryan S Renslow, Paul D Piehowski, Yehia M Ibrahim, Ryan T Kelly and Thomas O Metz

Analyst (London), Vol.144(3), pp.794-87

01/28/2019

DOI: https://doi.org/10.1039/c8an01574k

Handle:

https://hdl.handle.net/2376/107854

PMCID: PMC6349538

PMID: 30507980

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Abstract

Single-Cell Analysis - methods

Humans

Proteomics - methods

Genomics - methods

Mass Spectrometry - methods

Metabolomics - methods

Systems Biology

Precision Medicine

Mass-spectrometry based omics technologies - namely proteomics, metabolomics and lipidomics - have enabled the molecular level systems biology investigation of organisms in unprecedented detail. There has been increasing interest for gaining a thorough, functional understanding of the biological consequences associated with cellular heterogeneity in a wide variety of research areas such as developmental biology, precision medicine, cancer research and microbiome science. Recent advances in mass spectrometry (MS) instrumentation and sample handling strategies are quickly making comprehensive omics analyses of single cells feasible, but key breakthroughs are still required to push through remaining bottlenecks. In this review, we discuss the challenges faced by single cell MS-based omics analyses and highlight recent technological advances that collectively can contribute to comprehensive and high throughput omics analyses in single cells. We provide a vision of the potential of integrating pioneering technologies such as Structures for Lossless Ion Manipulations (SLIM) for improved sensitivity and resolution, novel peptide identification tactics and standards free metabolomics approaches for future applications in single cell analysis.

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Title: New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells
Creators: Sneha P Couvillion - Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA. thomas.metz@pnnl.gov
Ying Zhu
Gabe Nagy
Joshua N Adkins
Charles Ansong
Ryan S Renslow
Paul D Piehowski
Yehia M Ibrahim
Ryan T Kelly
Thomas O Metz
Publication Details: Analyst (London), Vol.144(3), pp.794-87
Academic Unit: Chemical Engineering and Bioengineering, School of
Publisher: England
Grant note: U2C ES030170 / NIEHS NIH HHS R33 CA225248 / NCI NIH HHS R03 CA222443 / NCI NIH HHS R21 EB020976 / NIBIB NIH HHS UC4 DK108101 / NIDDK NIH HHS U01 HL122703 / NHLBI NIH HHS U19 AI106772 / NIAID NIH HHS
Identifiers: 99900547107501842
Language: English
Resource Type: Journal article