Journal article
Nitrate Reduction with Halomonas campisalis: Kinetics of Denitrification at pH 9 and 12.5% NaCl
Water research (Oxford), Vol.35(17), pp.4237-4242
2001
Handle:
https://hdl.handle.net/2376/115622
PMID: 11791855
Abstract
Regeneration of ion exchange resins with NaCl produces brine containing high concentrations of nitrate that can be difficult to remove using standard biological, physical, or chemical technologies. In this study,
Halomonas campisalis (ATCC #700597) (Mormile
et al., 1999) was shown to completely reduce nitrate at 125
g/L NaCl and pH 9. This organism was also used in experiments to determine nitrate-reduction rates and biomass yields. Kinetic parameters were measured separately with glycerol, lactate, acetate, ethanol, and methanol. The specific nitrate-reduction rate coefficient was highest in cultures amended with acetate, while lactate and glycerol (a natural osmoticum in hypersaline environments) had lower reduction rates. No evidence of nitrate reduction was observed when ethanol or methanol was provided as an electron donor. Kinetic modeling provided values for nitrate and nitrite-reduction rate coefficients and for biomass yields. Measured rates and yields were similar to reported parameters obtained from non-halophilic nitrate-reducing cultures under low salt concentrations. Therefore, for highly saline solutions, the use of halophiles to selectively remove nitrate from these brines may represent a viable treatment option.
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Details
- Title
- Nitrate Reduction with Halomonas campisalis: Kinetics of Denitrification at pH 9 and 12.5% NaCl
- Creators
- Brent M Peyton - Center for Multiphase Environmental Research, Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USAMelanie R Mormile - Department of Biological Sciences, University of Missouri-Rolla, Rolla, MO 65409-1120, USAJames N Petersen - Center for Multiphase Environmental Research, Department of Chemical Engineering, Washington State University, Pullman, WA 99164-2710, USA
- Publication Details
- Water research (Oxford), Vol.35(17), pp.4237-4242
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900548059101842
- Language
- English
- Resource Type
- Journal article