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Nitrate promotes the transfer of methane-derived carbon from the methanotroph Methylobacter sp. to the methylotroph Methylotenera sp. in eutrophic lake water8.83 MB
CC BY V4.0, Open Access
Journal article
Nitrate promotes the transfer of methane-derived carbon from the methanotroph Methylobacter sp. to the methylotroph Methylotenera sp. in eutrophic lake water
Limnology and oceanography, Vol.66(3), pp.878-891
03/01/2021
Handle:
https://hdl.handle.net/2376/123834
Abstract
Eutrophic lakes are major contributors to global aquatic methane emissions. Methanotrophy, performed by methane oxidizing bacteria, results in the production of biomass, fermentation products and/or CO2, making methane-derived carbon available to non-methanotrophic organisms. Methanotrophs can co-occur with methylotrophs which are expected to consume methane-derived carbon. However, it is unknown if this interaction requires cell-to-cell contact, whether physicochemical factors affect this interaction, and what role this interaction may play in ecosystems and biogeochemical cycling in lakes. Here, we performed incubations of an enrichment culture obtained from a eutrophic lake with C-13-labeled methane, revealing the transfer of methane-derived carbon from the methanotroph Methylobacter sp. to a methylotroph of the genus Methylotenera. These microorganisms occurred both in mixed clusters and as single cells, indicating that their interaction does not require physical cell contact. In addition, the carbon transfer between the partners is dependent on the presence of nitrate, which is potentially used by Methylotenera sp. and in turn may affect the methane oxidation rate of Methylobacter sp. This interaction, and its dependence on nitrate, may have important implications for the carbon cycle in eutrophic lakes worldwide.
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Details
- Title
- Nitrate promotes the transfer of methane-derived carbon from the methanotroph Methylobacter sp. to the methylotroph Methylotenera sp. in eutrophic lake water
- Creators
- Sigrid van Grinsven - Royal Netherlands Institute for Sea ResearchJaap S Sinninghe Damste - NIOZ Royal Netherlands Inst Sea Res, Dept Marine Microbiol & Biogeochem, Den Burg, NetherlandsJohn Harrison - Washington State University VancouverLubos Polerecky - Utrecht UniversityLaura Villanueva - Utrecht University
- Publication Details
- Limnology and oceanography, Vol.66(3), pp.878-891
- Academic Unit
- Environment, School of the (CAS); Harrison Research Group: Global Change and Watershed Biochemistry
- Publisher
- WILEY
- Number of pages
- 14
- Grant note
- Netherlands Organization for Scientific Research (NWO); Netherlands Organization for Scientific Research (NWO) 024.002.002 / Soehngen Institute of Anaerobic Microbiology (SIAM) Gravitation grant of the Netherlands Ministry of Education, Culture and Science (OCW) 175.010.2009.011 / NWO; Netherlands Organization for Scientific Research (NWO)
- Identifiers
- 99900668012801842
- Language
- English
- Resource Type
- Journal article