Journal article
Nitrous oxide antinociception in BXD recombinant inbred mouse strains and identification of quantitative trait loci
Brain research, Vol.725(1), pp.23-29
1996
Handle:
https://hdl.handle.net/2376/116464
PMID: 8828582
Abstract
Among inbred mouse strains, DBA/2 mice are unique because of their poor responsiveness to nitrous oxide (N
2O) antinociception. As a first step towards identifying candidate genes involved in determining antinociceptive responsiveness to N
2O, male mice from the DBA/2 strain, the more responsive C57BL/6 strain, their B6D2F
1 offspring, and 22 BXD recombinant inbred (RI) strains derived from DBA/2 and C57BL/6 mice were exposed to N20 and evaluated using the acetic acid abdominal constriction test. When exposed to 70% N
2O, C57BL/6, DBA/2 and B6D2F
1 mice exhibited antinociceptive responses of 78, 22 and 55%, respectively. The BXD RI strains demonstrated varying degrees of responsiveness to N
2O. Cluster analysis revealed one cluster of 16 strains approximating the C57BL/6 progenitor (61.9–100% antinociceptive response to 70% N
2O) and another of six strains around the DBA/2 progenitor (9.1–40% antinociceptive response to 70% N
2O). The robust strain differences permitted screening the strain means with 1492 marker loci previously mapped in BXD RI strains. Using a QTL analysis specifically tailored to existing mouse RI strains, we found associations at the 0.01 level on seven chromosomes with the most promising marker loci being
Il2ra, Hbb, Hmgl rs7 and Gsl5 on chromosomes 2, 7, 16 and 19, respectively (
P < 0.002).
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Details
- Title
- Nitrous oxide antinociception in BXD recombinant inbred mouse strains and identification of quantitative trait loci
- Creators
- Raymond M Quock - Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, 1601 Parkview Avenue, Rockford, IL 61107-1897, USAJanet L Mueller - Department of Biomedical Sciences, University of Illinois College of Medicine at Rockford, 1601 Parkview Avenue, Rockford, IL 61107-1897, USALinda K Vaughn - Department of Basic Sciences, Marquette University School of Dentistry, Milwaukee, WI 53233-2188, USAJohn K Belknap - Research Service, Veterans Affairs Medical Center, Portland, OR 97201-2964, USA
- Publication Details
- Brain research, Vol.725(1), pp.23-29
- Academic Unit
- Psychology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900548303501842
- Language
- English
- Resource Type
- Journal article