Nitrous oxide-antinociception is mediated by opioid receptors and nitric oxide in the periaqueductal gray region of the midbrain

Dimitris E Emmanouil; Andrea S Dickens; Rick W Heckert; Yusuke Ohgami; Eunhee Chung; Shujie Han; Raymond M Quock

doi:10.1016/j.euroneuro.2007.06.008

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Nitrous oxide-antinociception is mediated by opioid receptors and nitric oxide in the periaqueductal gray region of the midbrain

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Nitrous oxide-antinociception is mediated by opioid receptors and nitric oxide in the periaqueductal gray region of the midbrain

Dimitris E Emmanouil, Andrea S Dickens, Rick W Heckert, Yusuke Ohgami, Eunhee Chung, Shujie Han and Raymond M Quock

European neuropsychopharmacology, Vol.18(3), pp.194-199

03/2008

DOI: https://doi.org/10.1016/j.euroneuro.2007.06.008

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https://hdl.handle.net/2376/113175

PMCID: PMC2279102

PMID: 17683915

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https://doi.org/10.1016/j.euroneuro.2007.06.008View

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Abstract

Antinociception

Periaqueductal gray

Nitrous oxide

Opioid receptors

Nitric oxide

Previous studies have shown that nitrous oxide (N2O)-induced antinociception is sensitive to antagonism by blockade of opioid receptors and also by inhibition of nitric oxide (NO) production. The present study was conducted to determine whether these occur within the same brain site. Mice were stereotaxically implanted with microinjection cannulae in the periaqueductal gray (PAG) area of the midbrain. In saline-pretreated mice, exposure to 70% N2O resulted in a concentration-dependent antinociceptive effect in the mouse abdominal constriction test. Pretreatment with an opioid antagonist in the PAG significantly antagonized the antinociceptive effect. Pretreatment with an inhibitor of NO production in the PAG also significantly antagonized the antinociceptive effect. These findings suggest that N2O acts in the PAG via an NO-dependent, opioid receptor-mediated mechanism to induce antinociception.

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Title: Nitrous oxide-antinociception is mediated by opioid receptors and nitric oxide in the periaqueductal gray region of the midbrain
Creators: Dimitris E Emmanouil - Department of Pediatric Dentistry, School of Dentistry, University of Athens, Athens, Greece
Andrea S Dickens - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
Rick W Heckert - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
Yusuke Ohgami - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
Eunhee Chung - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
Shujie Han - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
Raymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, P.O. Box 646534, Pullman, WA 99164-6534, USA
Publication Details: European neuropsychopharmacology, Vol.18(3), pp.194-199
Academic Unit: Psychology, Department of
Publisher: Elsevier B.V
Identifiers: 99900547966901842
Language: English
Resource Type: Journal article