Nitrous oxide-induced NO-dependent neuronal release of β-endorphin from the rat arcuate nucleus and periaqueductal gray

Yusuke Ohgami; Eunhee Chung; Raymond M Quock

doi:10.1016/j.brainres.2010.10.010

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Nitrous oxide-induced NO-dependent neuronal release of β-endorphin from the rat arcuate nucleus and periaqueductal gray

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Nitrous oxide-induced NO-dependent neuronal release of β-endorphin from the rat arcuate nucleus and periaqueductal gray

Yusuke Ohgami, Eunhee Chung and Raymond M Quock

Brain research, Vol.1366, pp.38-43

2010

DOI: https://doi.org/10.1016/j.brainres.2010.10.010

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https://hdl.handle.net/2376/105576

PMCID: PMC2993853

PMID: 20937263

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Abstract

Periaqueductal gray

Microdialysis

Rat

Nitric oxide

Arcuate nucleus

Nitrous oxide

β-endorphin

Nitrous oxide (N 2O)-induced antinociception is thought to result from nitric oxide (NO)-dependent neuronal release of endogenous opioid peptides in the central nervous system. The present study employed microdialysis to determine whether exposure to N 2O stimulates proopiomelanocortin (POMC) neurons to release β-endorphin in the arcuate nucleus (ARC) of the hypothalamus and the periaqueductal gray (PAG) of the midbrain. Male Sprague–Dawley rats were stereotaxically implanted with microdialysis probes in the ARC or PAG. Exposure to 70% N 2O significantly increased dialysate levels of oxidation products of NO as well as β-endorphin, compared to levels in fractions collected under room air. These increases in the ARC and PAG were abolished by systemic pretreatment with l-N G-nitro arginine methyl ester ( l-NAME). These findings suggest an association between increased NO activity and the stimulated release of β-endorphin during exposure of rats to N 2O. ►N 2O increases extracellular levels of nitrite and nitrate in the ARC and PAG. ►N 2O increases extracellular levels of β-endorphin in the ARC and PAG. ►These increases are both reversed by pretreatment with a NOS-inhibitor.

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Title: Nitrous oxide-induced NO-dependent neuronal release of β-endorphin from the rat arcuate nucleus and periaqueductal gray
Creators: Yusuke Ohgami - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USA
Eunhee Chung - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USA
Raymond M Quock - Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, WA, USA
Publication Details: Brain research, Vol.1366, pp.38-43
Academic Unit: Psychology, Department of
Publisher: Elsevier B.V
Identifiers: 99900546968401842
Language: English
Resource Type: Journal article