Journal article
Non-dispersive phloem-protein bodies (NPBs) of Populus trichocarpa consist of a SEOR protein and do not respond to cell wounding and Ca2
PeerJ (San Francisco, CA), Vol.6(4), pp.e4665-e4665
2018
Handle:
https://hdl.handle.net/2376/106391
PMCID: PMC5909683
PMID: 29682428
Abstract
Differentiating sieve elements in the phloem of angiosperms produce abundant phloem-specific proteins before their protein synthesis machinery is degraded. These P-proteins initially form dense bodies, which disperse into individual filaments when the sieve element matures. In some cases, however, the dense protein agglomerations remain intact and are visible in functional sieve tubes as non-dispersive P-protein bodies, or NPBs. Species exhibiting NPBs are distributed across the entire angiosperm clade. We found that NPBs in the model tree,
Populus trichocarpa
, resemble the protein bodies described from other species of the order Malpighiales as they all consist of coaligned tubular fibrils bundled in hexagonal symmetry. NPBs of all Malpighiales tested proved unresponsive to sieve tube wounding and Ca
2+
. The
P. trichocarpa
NPBs consisted of a protein encoded by a gene that in the genome database of this species had been annotated as a homolog of
SEOR1
(sieve element occlusion-related 1) in
Arabidopsis
. Sequencing of the gene in our plants corroborated this interpretation, and we named the gene
PtSEOR1
. Previously characterized SEOR proteins form irregular masses of P-protein slime in functional sieve tubes. We conclude that a subgroup of these proteins is involved in the formation of NPBs at least in the Malpighiales, and that these protein bodies have no role in rapid wound responses of the sieve tube network.
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Details
- Title
- Non-dispersive phloem-protein bodies (NPBs) of Populus trichocarpa consist of a SEOR protein and do not respond to cell wounding and Ca2
- Creators
- Daniel L Mullendore - ,Timothy Ross-Elliott - ,Yan Liu - ,Hanjo H Hellmann - ,Eric H Roalson - ,Winfried S Peters - ,Michael Knoblauch - ,
- Publication Details
- PeerJ (San Francisco, CA), Vol.6(4), pp.e4665-e4665
- Academic Unit
- Plant Pathology, Department of; Biological Sciences, School of
- Publisher
- PeerJ Inc; San Francisco, USA
- Grant note
- NSF-IOS 1456682 / National Science Foundation (USA)
- Identifiers
- 99900547057801842
- Language
- English
- Resource Type
- Journal article