Non-host disease resistance response in pea (Pisum sativum) pods: Biochemical function of DRR206 and phytoalexin pathway localization

Herana Kamal Seneviratne; Doralyn S Dalisay; Kye-Won Kim; Syed G A Moinuddin; Hong Yang; Christopher M Hartshorn; Laurence B Davin; Norman G Lewis

doi:10.1016/j.phytochem.2014.10.013

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Non-host disease resistance response in pea (Pisum sativum) pods: Biochemical function of DRR206 and phytoalexin pathway localization

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Non-host disease resistance response in pea (Pisum sativum) pods: Biochemical function of DRR206 and phytoalexin pathway localization

Herana Kamal Seneviratne, Doralyn S Dalisay, Kye-Won Kim, Syed G A Moinuddin, Hong Yang, Christopher M Hartshorn, Laurence B Davin and Norman G Lewis

Phytochemistry (Oxford), Vol.113(C), pp.140-148

05/2015

DOI: https://doi.org/10.1016/j.phytochem.2014.10.013

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https://hdl.handle.net/2376/115218

PMID: 25457488

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Abstract

Furans - metabolism

Sesquiterpenes - chemistry

Sesquiterpenes - metabolism

Gene Expression Regulation

Lignans - metabolism

Peas - cytology

Pterocarpans - metabolism

Plant Proteins - genetics

Plant Proteins - chemistry

Pterocarpans - chemistry

Plant Diseases - genetics

Molecular Structure

Plant Proteins - metabolism

Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Peas - genetics

Disease Resistance - genetics

Peas - chemistry

Peas - metabolism

Continually exposed to potential pathogens, vascular plants have evolved intricate defense mechanisms to recognize encroaching threats and defend themselves. They do so by inducing a set of defense responses that can help defeat and/or limit effects of invading pathogens, of which the non-host disease resistance response is the most common. In this regard, pea (Pisum sativum) pod tissue, when exposed to Fusarium solani f. sp. phaseoli spores, undergoes an inducible transcriptional activation of pathogenesis-related genes, and also produces (+)-pisatin, its major phytoalexin. One of the inducible pathogenesis-related genes is Disease Resistance Response-206 (DRR206), whose role in vivo was unknown. DRR206 is, however, related to the dirigent protein (DP) family. In this study, its biochemical function was investigated in planta, with the metabolite associated with its gene induction being pinoresinol monoglucoside. Interestingly, both pinoresinol monoglucoside and (+)-pisatin were co-localized in pea pod endocarp epidermal cells, as demonstrated using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging. In addition, endocarp epidermal cells are also the site for both chalcone synthase and DRR206 gene expression. Taken together, these data indicate that both (+)-pisatin and pinoresinol monoglucoside function in the overall phytoalexin responses.

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Title: Non-host disease resistance response in pea (Pisum sativum) pods: Biochemical function of DRR206 and phytoalexin pathway localization
Creators: Herana Kamal Seneviratne - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Doralyn S Dalisay - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Kye-Won Kim - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Syed G A Moinuddin - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Hong Yang - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Christopher M Hartshorn - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Laurence B Davin - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA
Norman G Lewis - Institute of Biological Chemistry, Washington State University, Pullman, WA 99164-6340, USA. Electronic address: lewisn@wsu.edu
Publication Details: Phytochemistry (Oxford), Vol.113(C), pp.140-148
Academic Unit: Biological Chemistry, Institute of
Publisher: England
Identifiers: 99900547804701842
Language: English
Resource Type: Journal article