Journal article
Non-maternal transmission is the major mode of ovine lentivirus transmission in a ewe flock: A molecular epidemiology study
Infection, genetics and evolution, Vol.10(7), pp.998-1007
2010
Handle:
https://hdl.handle.net/2376/115268
PMID: 20601169
Abstract
Transmission of ovine progressive pneumonia virus (OPPV), a lentivirus of sheep, occurs through both maternal and non-maternal means. Currently, the contribution of each route to the overall flock OPPV prevalence is poorly understood since previous serological epidemiologic studies lacked the ability to accurately track routes of transmission within an infected flock. In this study, the amount of maternal OPP transmission was assessed in a naturally infected ewe flock by applying molecular analyses to proviral sequences derived from peripheral blood leukocytes of OPP positive dam–daughter pairs (
N
=
40). Both proviral envelope (
env) and long terminal repeat (LTR) sequences, separately and combined, were utilized in the following 2 sequence analysis methods: phylogenetic analysis and pairwise distance calculations. True maternal transmission events were defined as agreement in 2 out of the 2 sequence analysis methods. Using this criterion, proviral
env sequences resulted in a 14.3% maternal transmission frequency, and proviral LTR sequences resulted in a 10% maternal transmission frequency. Both proportions of maternal transmission varied significantly from equality (
P
<
0.0001). This indicates that the remaining 85.7–90% of daughters are infected via non-maternal transmission. This is also the first study to calculate the OPP proviral rate of change for the
env gene and LTR promoter. Accurately defining the routes of OPPV transmission provides critical epidemiological data supporting management intended to reduce flock transmission and viral dose.
Metrics
6 Record Views
Details
- Title
- Non-maternal transmission is the major mode of ovine lentivirus transmission in a ewe flock: A molecular epidemiology study
- Creators
- Liam E Broughton-Neiswanger - Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, WA 99164-7040, USAStephen N White - Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, WA 99164-7040, USADonald P Knowles - Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, WA 99164-7040, USAMichelle R Mousel - U.S. Department of Agriculture, Agricultural Research Service, U.S. Sheep Experiment Station, Dubois, ID 83423, USAGregory S Lewis - U.S. Department of Agriculture, Agricultural Research Service, U.S. Sheep Experiment Station, Dubois, ID 83423, USADavid R Herndon - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, WA 99164-6630, USALynn M Herrmann-Hoesing - Washington State University, Department of Veterinary Microbiology and Pathology, Pullman, WA 99164-7040, USA
- Publication Details
- Infection, genetics and evolution, Vol.10(7), pp.998-1007
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier B.V
- Identifiers
- 99900547648001842
- Language
- English
- Resource Type
- Journal article