Journal article
Nonadditive Expression of Homoeologous Genes Is Established Upon Polyploidization in Hexaploid Wheat
Genetics (Austin), Vol.181(3), pp.1147-1157
03/2009
Handle:
https://hdl.handle.net/2376/111404
PMCID: PMC2651049
PMID: 19104075
Abstract
Effects of polyploidy in allohexaploid wheat (
Triticum aestivum
L.) have primarily been ascribed to increases in coding sequence variation and potential to acquire new gene functions through mutation of redundant loci. However, regulatory variation that arises through new promoter and transcription factor combinations or epigenetic events may also contribute to the effects of polyploidization. In this study, gene expression was characterized in a synthetic
T. aestivum
line and the
T. turgidum
and
Aegilops tauschii
parents to establish a timeline for such regulatory changes and estimate the frequency of nonadditive expression of homoeologous transcripts in newly formed
T. aestivum
. Large-scale analysis of nonadditive gene expression was assayed by microarray expression experiments, where synthetic
T. aestivum
gene expression was compared to additive model values (mid-parent) calculated from parental
T. turgidum
and
Ae. tauschii
expression levels. Approximately 16% of genes were estimated to display nonadditive expression in synthetic
T. aestivum
. A certain fraction of the genes (2.9%) showed overdominance or underdominance. cDNA–single strand conformation polymorphism analysis was applied to measure expression of homoeologous transcripts and further verify microarray data. The results demonstrate that allopolyploidization,
per se
, results in rapid initiation of differential expression of homoeologous loci and nonadditive gene expression in
T. aestivum.
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Details
- Title
- Nonadditive Expression of Homoeologous Genes Is Established Upon Polyploidization in Hexaploid Wheat
- Creators
- Michael Pumphrey - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506 andJianfa Bai - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506 andDebbie Laudencia-Chingcuanco - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506 andOlin Anderson - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506 andBikram S Gill - Department of Plant Pathology, Kansas State University, Manhattan, Kansas 66506 and
- Publication Details
- Genetics (Austin), Vol.181(3), pp.1147-1157
- Academic Unit
- Crop and Soil Sciences, Department of
- Publisher
- Genetics Society of America
- Identifiers
- 99900547691601842
- Language
- English
- Resource Type
- Journal article