Journal article
Novel innate immune functions for galectin-1: galectin-1 inhibits cell fusion by Nipah virus envelope glycoproteins and augments dendritic cell secretion of proinflammatory cytokines
The Journal of immunology (1950), Vol.175(1), pp.413-420
07/01/2005
Handle:
https://hdl.handle.net/2376/104349
PMID: 15972675
Abstract
Galectin-1 (gal-1), an endogenous lectin secreted by a variety of cell types, has pleiotropic immunomodulatory functions, including regulation of lymphocyte survival and cytokine secretion in autoimmune, transplant disease, and parasitic infection models. However, the role of gal-1 in viral infections is unknown. Nipah virus (NiV) is an emerging pathogen that causes severe, often fatal, febrile encephalitis. The primary targets of NiV are endothelial cells. NiV infection of endothelial cells results in cell-cell fusion and syncytia formation triggered by the fusion (F) and attachment (G) envelope glycoproteins of NiV that bear glycan structures recognized by gal-1. In the present study, we report that NiV envelope-mediated cell-cell fusion is blocked by gal-1. This inhibition is specific to the Paramyxoviridae family because gal-1 did not inhibit fusion triggered by envelope glycoproteins of other viruses, including two retroviruses and a pox virus, but inhibited fusion triggered by envelope glycoproteins of the related Hendra virus and another paramyxovirus. The physiologic dimeric form of gal-1 is required for fusion inhibition because a monomeric gal-1 mutant had no inhibitory effect on cell fusion. gal-1 binds to specific N-glycans on NiV glycoproteins and aberrantly oligomerizes NiV-F and NiV-G, indicating a mechanism for fusion inhibition. gal-1 also increases dendritic cell production of proinflammatory cytokines such as IL-6, known to be protective in the setting of other viral diseases such as Ebola infections. Thus, gal-1 may have direct antiviral effects and may also augment the innate immune response against this emerging pathogen.
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Details
- Title
- Novel innate immune functions for galectin-1: galectin-1 inhibits cell fusion by Nipah virus envelope glycoproteins and augments dendritic cell secretion of proinflammatory cytokines
- Creators
- Ernest L Levroney - Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at University of California, Los Angeles, CA 90095, USAHector C AguilarJennifer A FulcherLuciana KohatsuKaren E PaceMabel PangKevin B GurneyLinda G BaumBenhur Lee
- Publication Details
- The Journal of immunology (1950), Vol.175(1), pp.413-420
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Publisher
- United States
- Grant note
- AI61824 / NIAID NIH HHS AI07323 / NIAID NIH HHS AI059051 / NIAID NIH HHS GM63281 / NIGMS NIH HHS R21 AI059051 / NIAID NIH HHS R01 AI060694 / NIAID NIH HHS R01 GM063281 / NIGMS NIH HHS AI06094 / NIAID NIH HHS T32 AI007323 / NIAID NIH HHS F31 AI061824 / NIAID NIH HHS
- Identifiers
- 99900546973001842
- Language
- English
- Resource Type
- Journal article