Journal article
Novel mating type-dependent transcripts at the mating type locus in Magnaporthe oryzae
Gene, Vol.403(1), pp.6-17
2007
Handle:
https://hdl.handle.net/2376/110502
PMID: 17881155
Abstract
The mating type locus (
MAT1) of
Magnaporthe oryzae has similar structural organization to
MAT in other ascomycetes and encodes the mating type genes
MAT1-1-1 with an alpha-box motif and
MAT1-2-1 with an HMG-box motif in the
MAT1-1 and
MAT1-2 idiomorphs, respectively. Sequence and expression analyses of the
MAT1 locus indicated a second open reading frame (ORF),
MAT1-1-2, in the
MAT1-1 idiomorph, and novel mating-type dependent ORFs (
MAT1-1-3 and
MAT1-2-2) at the locus. The
MAT1-1-3 ORF initiated within the
MAT1-1 idiomorph while the
MAT1-2-2 ORF initiated at the border of the
MAT1-2 idiomorph with both ORFs sharing most of their reading frames in the
MAT1 flanking region. This suggests that the encoded proteins (MAT1-1-3 and MAT1-2-2) should be similar in their primary structures but can be distinguished by distinct N-termini with amino acids of 1 and 32, respectively, in each mating type. A CT dinucleotide repeat, (CT)n, present in the upstream region of
MAT1-1-3, was polymorphic among the isolates.
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Details
- Title
- Novel mating type-dependent transcripts at the mating type locus in Magnaporthe oryzae
- Creators
- Masaki Kanamori - Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, JapanHana Kato - Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, JapanNobuko Yasuda - National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, JapanShinzo Koizumi - National Agricultural Research Center, Tsukuba, Ibaraki 305-8666, JapanTobin L Peever - Department of Plant Pathology, Washington State University, Pulluman, WA 99164-1067, USATakashi Kamakura - Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba 278-8510, JapanTohru Teraoka - Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, JapanTsutomu Arie - Laboratory of Plant Pathology, Tokyo University of Agriculture and Technology (TUAT), Fuchu, Tokyo 183-8509, Japan
- Publication Details
- Gene, Vol.403(1), pp.6-17
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547277901842
- Language
- English
- Resource Type
- Journal article