Journal article
Nuclear factor-κB inhibitor peptide inhibits spontaneous and interleukin-1β-induced sleep
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.279(2), pp.R404-R413
08/01/2000
Handle:
https://hdl.handle.net/2376/110287
PMID: 10938226
Abstract
Nuclear factor-κB (NF-κB) is a transcription factor that when activated promotes production of several sleep-promoting substances such as interleukin-1β (IL-1β), tumor necrosis factor-α, and nerve growth factor. Therefore, we hypothesized that inhibition of NF-κB activation would attenuate sleep. A NF-κB cell-permeable inhibitor peptide (IP) was injected intracerebroventricularly (5 and 50 μg for rats, 100 μg for rabbits). On a separate day, time-matched control injections of a cell-permeable inactive control peptide were done in the same animals. The 50-μg dose of IP in rats and the 100-μg dose in rabbits significantly inhibited non-rapid eye movement sleep and rapid eye movement sleep if administered during the light period. Moreover, pretreatment of rabbits with 100 μg of the IP 12 h before intracerebroventricular injection of IL-1β (10 ng) significantly attenuated IL-1β-induced sleep and febrile responses. The current data support the hypothesis that a brain cytokine network is involved in sleep regulation and that NF-κB is a crucial factor in physiological sleep regulation.
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Details
- Title
- Nuclear factor-κB inhibitor peptide inhibits spontaneous and interleukin-1β-induced sleep
- Creators
- Takeshi Kubota - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164-6520Tetsuya Kushikata - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164-6520Jidong Fang - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164-6520James M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, Washington State University College of Veterinary Medicine, Pullman, Washington 99164-6520
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.279(2), pp.R404-R413
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547259301842
- Language
- English
- Resource Type
- Journal article