Journal article
Nuclear factor-κB-like activity increases in murine cerebral cortex after sleep deprivation
American journal of physiology. Regulatory, integrative and comparative physiology, Vol.276(6), pp.R1812-R1818
06/01/1999
Handle:
https://hdl.handle.net/2376/116320
PMID: 10362764
Abstract
Several well-defined sleep regulatory substances, e.g., interleukin-1β, activate the heterodimeric transcription factor nuclear factor-κB (NF-κB). Several substances that inhibit sleep, e.g., interleukin-4, inhibit NF-κB activation. NF-κB activation promotes production of several additional substances thought to be involved in sleep regulation, e.g., nitric oxide. We investigated, therefore, whether there are diurnal rhythms of NF-κB activation in brain and changes in the activation after sleep deprivation. Mice were kept on a 12:12-h light-dark cycle. In one experiment, groups of mice were killed every 3 h across the 24-h cycle. In another experiment, mice were killed at 1500 after 6 h of sleep deprivation, and a group of control mice were killed at the same time. Nuclear proteins were extracted from each brain tissue sample, and NF-κB-like activity was determined with an electrophoretic mobility shift assay. In cerebral cortex, but not other areas of brain, there was a diurnal rhythm in NF-κB-like activation; highest levels were found during the light period. NF-κB-like activation was higher in cerebral cortex after sleep deprivation compared with values obtained from control mice. The results are consistent with the hypothesis that sleep regulation involves multiple gene events, some of which include enhanced production of sleep regulatory substances, the actions of which involve NF-κB activation.
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Details
- Title
- Nuclear factor-κB-like activity increases in murine cerebral cortex after sleep deprivation
- Creators
- Zutang Chen - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164Janos Gardi - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164Tetsuya Kushikata - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164Jidong Fang - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164James M Krueger - Department of Veterinary and Comparative Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164
- Publication Details
- American journal of physiology. Regulatory, integrative and comparative physiology, Vol.276(6), pp.R1812-R1818
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Identifiers
- 99900547405801842
- Language
- English
- Resource Type
- Journal article