Nuclease released by Verticillium dahliae is a signal for non-host resistance

Lee A Hadwiger; Keri Druffel; Jodi L Humann; Brenda K Schroeder

doi:10.1016/j.plantsci.2012.11.011

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Nuclease released by Verticillium dahliae is a signal for non-host resistance

Journal article

Peer reviewed

Nuclease released by Verticillium dahliae is a signal for non-host resistance

Lee A Hadwiger, Keri Druffel, Jodi L Humann and Brenda K Schroeder

Plant science (Limerick), Vol.201-202(1), pp.98-107

03/2013

DOI: https://doi.org/10.1016/j.plantsci.2012.11.011

Handle:

https://hdl.handle.net/2376/103332

PMID: 23352407

Abstract

Plant Immunity

Genes, Plant

Plant Diseases - immunology

Transcriptional Activation

Seeds - enzymology

Fusarium - drug effects

Peas - microbiology

Plant Diseases - microbiology

Pterocarpans - metabolism

Deoxyribonucleases - metabolism

Deoxyribonucleases - isolation & purification

Verticillium - enzymology

Pterocarpans - genetics

Antifungal Agents - metabolism

Deoxyribonucleases - pharmacology

Seeds - cytology

Antifungal Agents - pharmacology

Disease Resistance

Enzyme Assays

Fusarium - growth & development

Antifungal Agents - isolation & purification

Host-Pathogen Interactions

Spores, Fungal - metabolism

Peas - immunology

Verticillium - immunology

DNA Damage

Enzyme Activation

Fungal Proteins - pharmacology

Peas - genetics

Fungal Proteins - metabolism

A DNase released from the fungal pathogen of bean, Fusarium solani f. sp. phaseoli (Fsph), was previously shown to signal the activation of total disease resistance and activate pathogenesis-related (PR) genes in pea. Data in the current study which used the pea-endocarp model to research non-host resistance, indicated that DNase released by Verticillium dahliae (Vd), pathogenic on potato also has non-host resistance-inducing capabilities in peas. Other strains of Vd that release DNase are pathogenic on other plant species. DNase catalytic activity was also released from representative genera of other pathogenic fungi. Purified VdDNase induced pisatin and pea gene DRR49 (PR-10 gene) in pea endocarp tissue. VdDNase reduced the in vitro growth of Vd but completely inhibited that of F. solani f. sp. pisi (Fspi) and a Colletotrichum pathogen of potato. VdDNase (2 units) applied to pea endocarp tissue both broke resistance to Fsph and increased resistance to Fspi. Pea DNA damage generated both by the VdDNase enzyme and the intact Vd spores indicated that the host DNA alteration is a component of the non-host resistance response (innate immunity). These data support the previously reported inductive potential of fungal DNase and further implicate fungal DNases as signals in activating non-host resistance responses.

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Title: Nuclease released by Verticillium dahliae is a signal for non-host resistance
Creators: Lee A Hadwiger - Department of Plant Pathology, Washington State University, Pullman, WA 99164 6430, USA. chitosan@wsu.edu
Keri Druffel
Jodi L Humann
Brenda K Schroeder
Publication Details: Plant science (Limerick), Vol.201-202(1), pp.98-107
Academic Unit: Plant Pathology, Department of
Publisher: Ireland
Identifiers: 99900546620401842
Language: English
Resource Type: Journal article

Nuclease released by Verticillium dahliae is a signal for non-host resistance

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