Nucleosome structure and repair of N-methylpurines in the GAL1-10 genes of Saccharomyces cerevisiae

Shisheng Li; Michael J Smerdon

doi:10.1074/jbc.M206623200

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Nucleosome structure and repair of N-methylpurines in the GAL1-10 genes of Saccharomyces cerevisiae

Journal article

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Peer reviewed

Nucleosome structure and repair of N-methylpurines in the GAL1-10 genes of Saccharomyces cerevisiae

Shisheng Li and Michael J Smerdon

The Journal of biological chemistry, Vol.277(47), pp.44651-44659

11/22/2002

DOI: https://doi.org/10.1074/jbc.M206623200

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https://hdl.handle.net/2376/106057

PMID: 12244104

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Abstract

Galactose - metabolism

DNA, Fungal - genetics

Nucleosomes - chemistry

Purines - metabolism

Saccharomyces cerevisiae - genetics

Humans

Nucleosomes - metabolism

Nucleosomes - genetics

Saccharomyces cerevisiae Proteins - genetics

Bleomycin - metabolism

Saccharomyces cerevisiae - metabolism

DNA Methylation

Animals

DNA Repair

DNA, Fungal - metabolism

Glucose - metabolism

Genes, Fungal

Trans-Activators - metabolism

Culture Media - chemistry

Nucleosome structure and repair of N-methylpurines were analyzed at nucleotide resolution in the divergent GAL1-10 genes of intact yeast cells, encompassing their common upstream-activating sequence. In glucose cultures where genes are repressed, nucleosomes with fixed positions exist in regions adjacent to the upstream-activating sequence, and the variability of nucleosome positioning sharply increases with increasing distance from this sequence. Galactose induction causes nucleosome disruption throughout the region analyzed, with those nucleosomes close to the upstream-activating sequence being most striking. In glucose cultures, a strong correlation between N-methylpurine repair and nucleosome positioning was seen in nucleosomes with fixed positions, where slow and fast repair occurred in nucleosome core and linker DNA, respectively. Galactose induction enhanced N-methylpurine repair in both strands of nucleosome core DNA, being most dramatic in the clearly disrupted, fixed nucleosomes. Furthermore, N-methylpurines are repaired primarily by the Mag1-initiated base excision repair pathway, and nucleotide excision repair contributes little to repair of these lesions. Finally, N-methylpurine repair is significantly affected by nearest-neighbor nucleotides, where fast and slow repair occurred in sites between pyrimidines and purines, respectively. These results indicate that nucleosome positioning and DNA sequence significantly modulate Mag1-initiated base excision repair in intact yeast cells.

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Title: Nucleosome structure and repair of N-methylpurines in the GAL1-10 genes of Saccharomyces cerevisiae
Creators: Shisheng Li - Biochemistry and Biophysics, School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4660, USA
Michael J Smerdon
Publication Details: The Journal of biological chemistry, Vol.277(47), pp.44651-44659
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: ES04106 / NIEHS NIH HHS
Identifiers: 99900546711201842
Language: English
Resource Type: Journal article