Journal article
Null mutation of AtCUL1 causes arrest in early embryogenesis in Arabidopsis
Molecular biology of the cell, Vol.13(6), pp.1916-1928
2002
Handle:
https://hdl.handle.net/2376/109950
PMCID: PMC117614
PMID: 12058059
Abstract
The SCF (for SKP1, Cullin/CDC53, F-box protein) ubiquitin ligase targets a number of cell cycle regulators, transcription factors, and other proteins for degradation in yeast and mammalian cells. Recent genetic studies demonstrate that plant F-box proteins are involved in auxin responses, jasmonate signaling, flower morphogenesis, photocontrol. of circadian clocks, and leaf senescence, implying a large spectrum of functions for the SCF pathway in plant development. Here, we present a molecular and functional characterization of plant cullins. The Arabidopsis genome contains 11 cullin-related genes. Complementation assays revealed that AtCUL1 but not AtCUL4 can functionally complement the yeast cdc53 mutant. Arabidopsis mutants containing transfer DNA (T-DNA) insertions in the AtCUL1 gene were shown to display an arrest in early embryogenesis. Consistently, both the transcript and the protein of the AtCUL1 gene were found to accumulate in embryos. The AtCUL1 protein localized mainly in the nucleus but also weakly in the cytoplasm during interphase and colocalized with the mitotic spindle in metaphase. Our results demonstrate a critical role for the SCF ubiquitin ligase in Arabidopsis embryogenesis.
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Details
- Title
- Null mutation of AtCUL1 causes arrest in early embryogenesis in Arabidopsis
- Creators
- Wen-Hui Shen - Institut de biologie moléculaire des plantesYves Parmentier - Institut de biologie moléculaire des plantesHanjo Hellmann - University of Texas at AustinE Lechner - Institut de Biologie des PlantesAiwu Dong - Institut de biologie moléculaire des plantesJ Masson - Santé de la vigne et qualité du vinFabienne Granier - Laboratoire de biologie cellulaire et moléculaireLoic Lepiniec - Laboratoire de biologie cellulaire et moléculaireMarie Estelle - University of Texas at AustinPascal Genschik - Institut de biologie moléculaire des plantes
- Publication Details
- Molecular biology of the cell, Vol.13(6), pp.1916-1928
- Academic Unit
- Biological Sciences, School of
- Publisher
- American Society for Cell Biology
- Identifiers
- 99900547240401842
- Language
- English
- Resource Type
- Journal article