Journal article
ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery
The Journal of cell biology, Vol.183(2), pp.313-322
10/20/2008
Handle:
https://hdl.handle.net/2376/104006
PMCID: PMC2568026
PMID: 18852297
Abstract
Formation of flagellar outer dynein arms in Chlamydomonas reinhardtii requires the ODA16 protein at a previously uncharacterized assembly step. Here, we show that dynein extracted from wild-type axonemes can rebind to oda16 axonemes in vitro, and dynein in oda16 cytoplasmic extracts can bind to docking sites on pf28 (oda) axonemes, which is consistent with a role for ODA16 in dynein transport, rather than subunit preassembly or binding site formation. ODA16 localization resembles that seen for intraflagellar transport (IFT) proteins, and flagellar abundance of ODA16 depends on IFT. Yeast two-hybrid analysis with mammalian homologues identified an IFT complex B subunit, IFT46, as a directly interacting partner of ODA16. Interaction between Chlamydomonas ODA16 and IFT46 was confirmed through in vitro pull-down assays and coimmunoprecipitation from flagellar extracts. ODA16 appears to function as a cargo-specific adaptor between IFT particles and outer row dynein needed for efficient dynein transport into the flagellar compartment.
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Details
- Title
- ODA16 aids axonemal outer row dynein assembly through an interaction with the intraflagellar transport machinery
- Creators
- Noveera T Ahmed - Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210Chunlei Gao - Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210Ben F Lucker - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844Douglas G Cole - Department of Microbiology, Molecular Biology and Biochemistry, University of Idaho, Moscow, ID 83844David R Mitchell - Department of Cell and Developmental Biology, State University of New York Upstate Medical University, Syracuse, NY 13210
- Publication Details
- The Journal of cell biology, Vol.183(2), pp.313-322
- Academic Unit
- Center for Reproductive Biology
- Identifiers
- 99900547099801842
- Language
- English
- Resource Type
- Journal article