Journal article
OPDA Has Key Role in Regulating Plant Susceptibility to the Root-Knot Nematode Meloidogyne hapla in Arabidopsis
Frontiers in plant science, Vol.7(2016), pp.1565-1565
10/24/2016
Handle:
https://hdl.handle.net/2376/117347
PMCID: PMC5075541
PMID: 27822219
Abstract
Jasmonic acid (JA) is a plant hormone that plays important roles in regulating plant defenses against necrotrophic pathogens and herbivorous insects, but the role of JA in mediating the plant responses to root-knot nematodes has been unclear. Here we show that an application of either methyl jasmonate (MeJA) or the JA-mimic coronatine (COR) on
Arabidopsis
significantly reduced the number of galls caused by the root-knot nematode
Meloidogyne hapla
. Interestingly, the MeJA-induced resistance was independent of the JA-receptor COI1 (CORONATINE INSENSITIVE 1). The MeJA-treated plants accumulated the JA precursor
cis
-(+)-12-oxo-phytodienoic acid (OPDA) in addition to JA/JA-Isoleucine, indicating a positive feedback loop in JA biosynthesis. Using mutants in the JA-biosynthetic pathway, we found that plants deficient in the biosynthesis of JA and OPDA were hyper-susceptible to
M. hapla.
However, the
opr3
mutant, which cannot convert OPDA to JA, exhibited wild-type levels of nematode galling. In addition, mutants in the JA-biosynthesis and perception which lie downstream of
opr3
also displayed wild-type levels of galling. The data put OPR3 (OPDA reductase 3) as the branch point between hyper-susceptibility and wild-type like levels of disease. Overall, the data suggests that the JA precursor, OPDA, plays a role in regulating plant defense against nematodes.
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Details
- Title
- OPDA Has Key Role in Regulating Plant Susceptibility to the Root-Knot Nematode Meloidogyne hapla in Arabidopsis
- Creators
- Cynthia Gleason - Department of Plant Molecular Biology and Physiology, Georg August University - Albrecht von Haller InstituteNatthanon Leelarasamee - Department of Plant Molecular Biology and Physiology, Georg August University - Albrecht von Haller InstituteDorothea Meldau - Department of Plant Biochemistry, Georg August University - Albrecht von Haller InstituteIvo Feussner - Department of Plant Biochemistry, Georg August University - Albrecht von Haller Institute
- Publication Details
- Frontiers in plant science, Vol.7(2016), pp.1565-1565
- Academic Unit
- Plant Pathology, Department of
- Publisher
- Frontiers Media S.A
- Grant note
- GL729/1-1 / Deutsche Forschungsgemeinschaft
- Identifiers
- 99900548566001842
- Language
- English
- Resource Type
- Journal article