Journal article
Oleaginous yeast Yarrowia lipolytica mutants with a disrupted fatty acyl-CoA synthetase gene accumulate saturated fatty acid
Process biochemistry (1991), Vol.46(7), pp.1436-1441
2011
Handle:
https://hdl.handle.net/2376/118082
Abstract
Fatty acyl-CoA synthetases are critical enzymes involved in lipid metabolism. The oleaginous yeast
Yarrowia lipolytica is currently generating interest in biofuel research due to its ability to convert raw materials into value-added end products. In this study, the putative acyl-CoA synthetase gene
YAL1 in
Y. lipolytica was interrupted with the copper resistance (
CRF1) gene to allow selection without antibiotics to facilitate industrial applications. Deletion of
YAL1 led to reduced acyl-CoA synthetase activity. Furthermore, the fatty acid profile and lipid content of the mutant were different from the wild-type strain. The ratio of saturated to unsaturated fatty acids increased 6-fold, and the total lipid production of the mutant strain increased to 1.47-fold of the wild-type strain. The results indicate that
YAL1 in
Y. lipolytica is involved in fatty acid elongation and desaturation, whereas the homologous, highly conserved
FAA1 gene from
Saccharomyces cerevisiae was shown to be responsible for fatty acid activation. The increased ratio of saturated to unsaturated fatty acids would result in a higher combustion value and better oxidative stability for biofuel products obtained from the fatty acids from the engineered
Y. lipolytica mutant.
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Details
- Title
- Oleaginous yeast Yarrowia lipolytica mutants with a disrupted fatty acyl-CoA synthetase gene accumulate saturated fatty acid
- Creators
- Jinjing Wang - The Laboratory of Molecular Genetics and Breeding of Yeasts, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR ChinaBorun Zhang - The Laboratory of Molecular Genetics and Breeding of Yeasts, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR ChinaShulin Chen - BBEL, Department of Biological Systems Engineering, Washington State University, Pullman, WA 99163, USA
- Publication Details
- Process biochemistry (1991), Vol.46(7), pp.1436-1441
- Publisher
- Elsevier Ltd
- Identifiers
- 99900582328301842
- Language
- English
- Resource Type
- Journal article