Journal article
On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine
Bioresource technology, Vol.154, pp.282-289
02/2014
Handle:
https://hdl.handle.net/2376/112298
PMID: 24412480
Abstract
Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of two feedstocks, wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktails containing cellulase from T. reesei RUT-C30 and β-glucosidase from A. saccharolyticus. The sugar yields using the on-site enzyme cocktails were compared with commercial enzymes preparations, Celluclast 1.5L and Novozym 188 at two substrate concentrations, 5% and 10% (w/w) and enzyme loading at 5 and 15 FPU/g glucan for WECS and WELP. The highest sugar yields were obtained at 5% (w/w) substrate concentration and 15 FPU/g glucan for both feedstocks. Glucose yields of 81% and 88% were obtained from on-site and commercial enzymes, respectively using WECS as feed stock. The sugar yields were 55% and 58% for WELP samples hydrolyzed with on-site and commercial enzymes, respectively.
Metrics
6 Record Views
Details
- Title
- On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine
- Creators
- Vandana Rana - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United StatesAnahita D Eckard - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United StatesPhilip Teller - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United StatesBirgitte K Ahring - Bioproducts, Sciences and Engineering Laboratory (BSEL), Washington State University Richland, WA 99354, United States. Electronic address: bka@wsu.edu
- Publication Details
- Bioresource technology, Vol.154, pp.282-289
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- England
- Identifiers
- 99900547447801842
- Language
- English
- Resource Type
- Journal article