Journal article
One-pot bioinspired synthesis of all-inclusive protein-protein nanoflowers for point-of-care bioassay: detection of E. coli O157:H7 from milk
Nanoscale, Vol.8(45), pp.18980-18986
12/07/2016
Handle:
https://hdl.handle.net/2376/102638
PMID: 27808326
Abstract
Protein-protein conjugates play a vital role in bioassays with their inherent functions of biological recognition and signal amplification. Herein, a one-pot green method for synthesis of all-inclusive protein-protein nanoflowers has been developed. The protein-protein nanoflowers integrate both essential functions of biological recognition and signal amplification, and they were used as ideal signal labels for the sensitive point-of-care detection of Escherichia coli O157:H7. Especially noteworthy, the prepared Con A-invertase-CaHPO
hybrid nanoflowers simultaneously loaded sufficient invertase and enhanced the activity of the immobilized invertase, which fits well with the requirements of signal labels for bioassays. Due to the conversion of sucrose to glucose by invertase, Con A-invertase-CaHPO
hybrid nanoflowers were successfully used for the reliable point-of-care detection of food pathogens by a personal glucose meter. The presented approach successfully resolved the bottleneck in preparing protein-protein conjugate-based signal labels for bioassays using enzyme-based signal amplification strategies, which holds great promise to develop on-demand protein-protein conjugates for a variety of applications extending from biosensors and biomedicine to energy, environmental monitoring and remediation.
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Details
- Title
- One-pot bioinspired synthesis of all-inclusive protein-protein nanoflowers for point-of-care bioassay: detection of E. coli O157:H7 from milk
- Creators
- Ranfeng Ye - College of Chemistry and College of Life Sciences, Central China Normal University, Wuhan 430079, China. dan.du@mail.ccnu.edu.cnChengzhou ZhuYang SongJunhua SongShaofang FuQian LuXu YangMei-Jun ZhuDan DuHe LiYuehe Lin
- Publication Details
- Nanoscale, Vol.8(45), pp.18980-18986
- Academic Unit
- School of Mechanical and Materials Engineering; Department of Chemistry
- Publisher
- England
- Identifiers
- 99900546633501842
- Language
- English
- Resource Type
- Journal article