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Optical imaging of gastric cancer with near-infrared heptamethine carbocyanine fluorescence dyes6.36 MB
CC BY V4.0, Open Access
Journal article
Optical imaging of gastric cancer with near-infrared heptamethine carbocyanine fluorescence dyes
Oncotarget, Vol.7(35), pp.57277-57289
08/30/2016
PMCID: PMC5302988
PMID: 27329598
Abstract
Near-infrared fluorescence (NIRF) imaging agents are promising tools for noninvasive cancer imaging. Here, we explored the tumor-specific targeting ability of NIRF heptamethine carbocyanine MHI-148 dye in cultured gastric cancer cells, gastric cancer cell-derived and patient-derived tumor xenograft (PDX) models. We show that the NIRF dye specifically accumulated in tumor regions of both xenograft models, suggesting the potential utility of the dye for tumor-specific imaging and targeting in gastric cancer. We also demonstrated significant correlations between NIRF signal intensity and tumor volume in PDX models. Mechanistically, the higher cellular uptake of MHI-148 in gastric cancer cells than in normal cells was stimulated by hypoxia and activation of a group of organic anion-transporting polypeptide (OATP) genes. Importantly, this NIRF dye was not retained in inflammatory stomach tissues induced by gastric ulcer in mice. In addition, fresh clinical gastric tumor specimens, when perfused with NIR dye, exhibited increased uptake of NIR dye in situ. Together, these results show the possibility of using NIRF dyes as novel candidate agents for clinical imaging and detection of gastric cancer.
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Details
- Title
- Optical imaging of gastric cancer with near-infrared heptamethine carbocyanine fluorescence dyes
- Creators
- Ningning Zhao - Fourth Military Medical UniversityCaiqin Zhang - Fourth Military Medical UniversityYong Zhao - Laboratory Animal Center, the Fourth Military Medical University, Xi'an, Shaanxi 710032, ChinaBing Bai - Fourth Military Medical UniversityJiaze An - Fourth Military Medical UniversityHai Zhang - Laboratory Animal Center, the Fourth Military Medical University, Xi'an, Shaanxi 710032, ChinaJason Boyang Wu - Washington State University, Pharmaceutical Sciences, Department ofChanghong Shi - Cedars-Sinai Medical Center
- Publication Details
- Oncotarget, Vol.7(35), pp.57277-57289
- Academic Unit
- Pharmacy and Pharmaceutical Sciences, College of
- Identifiers
- 99900620489401842
- Language
- English
- Resource Type
- Journal article