Journal article
Optimization and evaluation of heat-shock condition for spore enumeration being used in thermal-process verification: Differential responses of spores and vegetative cells of Clostridium sporogenes to heat shock
Food science and biotechnology, Vol.20(3), pp.751-757
06/2011
Handle:
https://hdl.handle.net/2376/111999
Abstract
To evaluate a heat-shock condition for the enumeration of Clostridium sporogenes spores, a surrogate for C. botulinum spores, we examined the heat tolerance of C. sporogenes spores and vegetative cells exposed to a heat shock at 90°C. From the D values of the spores determined in the temperature range of 113–121°C, z value (±SD) and D90°C value were estimated to be 10.16±0.90°C and 1,071.52 min, respectively, and the inactivation rates were predicted to be only approximately 2% at 90°C for up to 10 min. Meanwhile, the viable count of spores was significantly higher when activated under a heat-shock condition of 90°C for over 9 min than those activated for shorter time periods. The heat tolerance of vegetative cells was extremely low, showing a D90°C value (±SD) of 0.21±0.01 min. Finally, 3 different heat-shock conditions were compared: 70°C for 30 min, 80°C for 20 min, and 90°C for 10 min, and the experimental comparative data showed no significant differences in viable spore counts. Consequently, these results support that the heat-shock treatment at 90°C for 10 min is suitable to activate spores and to inactivate vegetative cells of C. sporogenes.
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Details
- Title
- Optimization and evaluation of heat-shock condition for spore enumeration being used in thermal-process verification: Differential responses of spores and vegetative cells of Clostridium sporogenes to heat shock
- Creators
- Bo Byun - Department of Food and Biotechnology Korea University Jochiwon, Chungnam 339-700 KoreaHong-Yon Cho - Department of Food and Biotechnology Korea University Jochiwon, Chungnam 339-700 KoreaHan-Joon Hwang - Department of Food and Biotechnology Korea University Jochiwon, Chungnam 339-700 KoreaJae-Hyung Mah - Department of Food and Biotechnology Korea University Jochiwon, Chungnam 339-700 KoreaYanhong Liu - College of Engineering China Agricultural University Beijing 100083 ChinaJuming Tang - Department of Biological Systems Engineering Washington State University Pullman WA 99164-6120 USADong-Hyun Kang - Department of Agricultural Biotechnology Seoul National University Seoul 151-921 Korea
- Publication Details
- Food science and biotechnology, Vol.20(3), pp.751-757
- Academic Unit
- Biological Systems Engineering, Department of; Plant Pathology, Department of
- Publisher
- The Korean Society of Food Science and Technology; Heidelberg
- Identifiers
- 99900547705201842
- Language
- English
- Resource Type
- Journal article