Optimized Reconstruction Techniques for Multiplexed Dual-Gate Ion Mobility Mass Spectrometry Experiments

Austen L Davis; Tobias Reinecke; Kelsey A Morrison; Brian H Clowers

doi:10.1021/acs.analchem.8b04175

Optimized Reconstruction Techniques for Multiplexed Dual-Gate Ion Mobility Mass Spectrometry Experiments

Austen L Davis, Tobias Reinecke, Kelsey A Morrison Brian H Clowers

Analytical chemistry (Washington), Vol.91(2), pp.1432-1440

01/15/2019

: https://doi.org/10.1021/acs.analchem.8b04175

https://hdl.handle.net/2376/101633

: 30561982

When coupling drift-tube gas-phase ion mobility separations with ion trapping mass analyzers an integrative, stepped approach to spectral reconstruction is a logical, yet highly inefficient means to determine gas-phase mobility coefficients. This experimental mode is largely predicated on the respective time scales of the two techniques each requiring tens of milliseconds to complete under routine conditions. Multiplexing techniques, such as Fourier and Hadamard based techniques, are a potential solution but still require extended experimental times that are not fully compatible with modern front-end separation schemes. Using a basis pursuit denoising (BPDN) approach to deconvolute Fourier transform ion mobility mass spectrometry (FT-IMMS) drift time spectra, we demonstrate significant time savings while maintaining a high degree of spectral resolution and signal-to-noise ratio. Under ideal conditions, the FT-IMMS operates with increased ion transmission (up to 25%); however, the linear chirp that spans into the kHz range often leads to significant levels of ion gate depletion, which limit both resolving power and ion transmission. The method proposed in this manuscript demonstrates the potential to reduce IMS acquisition time while simultaneously maximizing spectral resolution at longer effective gate pulse widths compared to the traditional set of multiplexing and signal averaging experiments.

: Optimized Reconstruction Techniques for Multiplexed Dual-Gate Ion Mobility Mass Spectrometry Experiments
: Austen L Davis - Department of Chemistry , Washington State University , Pullman , Washington 99164 , United States
Tobias Reinecke - Department of Chemistry , Washington State University , Pullman , Washington 99164 , United States
Kelsey A Morrison - Department of Chemistry , Washington State University , Pullman , Washington 99164 , United States
Brian H Clowers - Department of Chemistry , Washington State University , Pullman , Washington 99164 , United States
: Analytical chemistry (Washington), Vol.91(2), pp.1432-1440
: Chemistry, Department of
: United States
: 99900546628401842
: English
: Journal article