Journal article
Organization and Regulation of Pentachlorophenol-Degrading Genes in Sphingobium chlorophenolicum ATCC 39723
Journal of bacteriology, Vol.184(17), pp.4672-4680
09/2002
Handle:
https://hdl.handle.net/2376/104436
PMCID: PMC135293
PMID: 12169590
Abstract
The first three enzymes of the pentachlorophenol (PCP) degradation pathway in
Sphingobium chlorophenolicum
(formerly
Sphingomonas chlorophenolica
) ATCC 39723 have been characterized, and the corresponding genes,
pcpA
,
pcpB
, and
pcpC
, have been individually cloned and sequenced. To search for new genes involved in PCP degradation and map the physical locations of the
pcp
genes, a 24-kb fragment containing
pcpA
and
pcpC
was completely sequenced. A putative LysR-type transcriptional regulator gene,
pcpM
, and a maleylacetate reductase gene,
pcpE
, were identified upstream of
pcpA. pcpE
was found to play a role in PCP degradation.
pcpB
was not found on the 24-kb fragment. The four gene products PcpB, PcpC, PcpA, and PcpE were responsible for the metabolism of PCP to 3-oxoadipate in ATCC 39723, and inactivational mutation of each gene disrupted the degradation pathway. The organization of the
pcp
genes is unusual because the four PCP-degrading genes,
pcpA
,
pcpB
,
pcpC
, and
pcpE
, were found to be located at four discrete locations. Two hypothetical LysR-type regulator genes,
pcpM
and
pcpR,
have been identified;
pcpM
was not required, but
pcpR
was essential for the induction of
pcpB
,
pcpA
, and
pcpE
. The coinducers of PcpR were PCP and other polychlorinated phenols. The expression of
pcpC
was constitutive. Thus, the organization and regulation of the genes involved in PCP degradation to 3-oxoadipate were documented.
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Details
- Title
- Organization and Regulation of Pentachlorophenol-Degrading Genes in Sphingobium chlorophenolicum ATCC 39723
- Creators
- Mian Cai - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4233Luying Xun - School of Molecular Biosciences, Washington State University, Pullman, Washington 99164-4233
- Publication Details
- Journal of bacteriology, Vol.184(17), pp.4672-4680
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900547098901842
- Language
- English
- Resource Type
- Journal article