Osmoregulatory effects of hypophysectomy and homologous prolactin replacement in hybrid striped bass

Leslie F Jackson; Stephen D McCormick; Steffen S Madsen; Penny Swanson; Craig V Sullivan

doi:10.1016/j.cbpc.2004.10.004

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Journal article

Osmoregulatory effects of hypophysectomy and homologous prolactin replacement in hybrid striped bass

Leslie F Jackson, Stephen D McCormick, Steffen S Madsen, Penny Swanson and Craig V Sullivan

Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol.140(2), pp.211-218

02/2005

DOI: https://doi.org/10.1016/j.cbpc.2004.10.004

Handle:

https://hdl.handle.net/2376/116752

PMID: 15649768

Abstract

Branchial Region - drug effects

Bass - blood

Adenosine Triphosphatases - metabolism

Water-Electrolyte Balance - drug effects

Branchial Region - enzymology

Prolactin - pharmacology

Electrolytes - blood

Injections

Animals

Osmolar Concentration

Bass - physiology

Bass - metabolism

Hypophysectomy

Prolactin - administration & dosage

The effects of ovine prolactin (oPRL) and striped bass prolactin (sbPRL; Morone saxatilis) on plasma osmolality, electrolyte balance, and gill Na(+),K(+)-ATPase activity were investigated in hypophysectomized (Hx), freshwater (FW)-acclimated, hybrid striped bass (M. saxatilisxMorone chrysops). They were kept in dilute (isoosmotic) seawater for about 10 days after surgery. Seven days after transfer to FW, Hx fish had lower plasma osmolality and lower levels of Na(+), Cl(-), and Ca(2+) than sham-operated and intact fish. Fish were injected four times with oPRL (1, 5, or 20 microg/g body mass), sbPRL (10 or 100 ng/g), or hormone vehicle (0.9% NaCl) at 48-h intervals (days 0, 2, 4, and 6) in FW and then sampled for blood plasma 24 h after the fourth injection (day 7). In Hx fish, oPRL (5 and 20 microg/g) and sbPRL (10 and 100 ng/g) were effective in maintaining plasma osmolality and levels of Na(+), Cl(-), and Ca(2+) above values seen in saline-injected controls. Hypophysectomy did not affect branchial Na(+),K(+)-ATPase activity, but enzyme activity was significantly reduced in Hx fish receiving oPRL (20 mug/g) or sbPRL (10 or 100 ng/g). These results indicate that PRL acts to maintain plasma osmotic and ionic balance in FW-adapted hybrid striped bass, and that this may involve downregulation of branchial Na(+),K(+)-ATPase activity.

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Title: Osmoregulatory effects of hypophysectomy and homologous prolactin replacement in hybrid striped bass
Creators: Leslie F Jackson - Department of Zoology, North Carolina State University, Raleigh, NC 27695-7617, USA. antonop@hua.gr
Stephen D McCormick
Steffen S Madsen
Penny Swanson
Craig V Sullivan
Publication Details: Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, Vol.140(2), pp.211-218
Academic Unit: UNKNOWN
Publisher: England
Identifiers: 99900547714901842
Language: English
Resource Type: Journal article

Osmoregulatory effects of hypophysectomy and homologous prolactin replacement in hybrid striped bass

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