Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping

Mangal Roy; Susmita Bose

doi:10.1002/jbm.a.34181

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Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping

Journal article

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Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping

Mangal Roy and Susmita Bose

Journal of biomedical materials research. Part A, Vol.100(9), pp.2450-2461

09/2012

DOI: https://doi.org/10.1002/jbm.a.34181

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https://hdl.handle.net/2376/105372

PMCID: PMC3430374

PMID: 22566212

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Abstract

Cell Line

RANK Ligand - metabolism

Calcium Phosphates - chemistry

Magnesium - metabolism

Osteoclasts - cytology

Strontium - metabolism

Cell Adhesion

Osteoclasts - metabolism

Strontium - chemistry

Bone Substitutes - metabolism

Magnesium - chemistry

Animals

Cell Differentiation

Mice

Calcium Phosphates - metabolism

Bone Substitutes - chemistry

Bone substitute materials are required to support the remodeling process, which consists of osteoclastic resorption and osteoblastic synthesis. Osteoclasts, the bone-resorbing cells, generate from differentiation of hemopoietic mononuclear cells. In the present study, we have evaluated the effects of 1.0 wt % strontium (Sr) and 1.0 wt % magnesium (Mg) doping in beta-tricalcium phosphate (β-TCP) on the differentiation of mononuclear cells into osteoclast-like cells and its resorptive activity. In vitro osteoclast-like cell formation, adhesion, and resorption were studied using osteoclast precursor RAW 264.7 cell, supplemented with receptor activator of nuclear factor κβ ligand (RANKL). Osteoclast-like cell formation was noticed on pure and Sr-doped β-TCP samples at day 8, which was absent on Mg-doped β-TCP samples indicating decrease in initial osteoclast differentiation due to Mg doping. After 21 days of culture, osteoclast-like cell formation was evident on all samples with osteoclastic markers such as actin ring, multiple nuclei, and presence of vitronectin receptor α(v)β(3) integrin. After osteoclast differentiation, all substrates showed osteoclast-like cell-mediated degradation, however, significantly restricted for Mg-doped β-TCP samples. Our present results indicated that substrate chemistry controlled osteoclast differentiation and resorptive activity, which can be used in designing TCP-based resorbable bone substitutes with controlled degradation properties.

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Title: Osteoclastogenesis and osteoclastic resorption of tricalcium phosphate: effect of strontium and magnesium doping
Creators: Mangal Roy - W M Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164, USA
Susmita Bose
Publication Details: Journal of biomedical materials research. Part A, Vol.100(9), pp.2450-2461
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Grant note: R01 EB007351 / NIBIB NIH HHS N1H-R01-EB-007351 / NIBIB NIH HHS
Identifiers: 99900546661901842
Language: English
Resource Type: Journal article