Journal article
Ovarian uptake of vitellogenin and another very high density lipoprotein in winter flounder (Pseudopleuronectes americanus) and their relationship with yolk proteins
Biochemistry and cell biology, Vol.68(1), pp.330-335
01/01/1990
Handle:
https://hdl.handle.net/2376/110935
Abstract
Ultracentrifugation analysis of female winter flounder plasma confirmed that in addition to vitellogenin another different lipoprotein is found as very high density lipoprotein (VHDL). Accordingly this protein (formerly known as peak A protein) has been named VHDL II, with respect to vitellogenin which we designate VHDL I. Both these proteins were shown to be taken up in vivo by the ovary of vitellogenic females. Based on total mass, vitellogenin and VHDL II could potentially contribute in similar amounts to yolk protein accumulated by developing oocytes. Sodium dodecyl sulfate – polyacrylamide gel electrophoresis of vitellogenic oocyte yolk proteins revealed five major protein subunits of 101.4, 94.4, 68.7, 25.5, and 22.5 kilodaltons (kDa). Western blots of these yolk protein subunits established three of them (104.4, 94.4, and 22.5 kDa) as originating from vitellogenin. Similar Western blots utilizing a VHDL II antisera identified the 68.7-kDa yolk protein subunit as arising from incorporated serum VHDL II.Key words: VHDL II, vitellogenin, oocyte yolk proteins, in vivo uptake, Western blot.
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Details
- Title
- Ovarian uptake of vitellogenin and another very high density lipoprotein in winter flounder (Pseudopleuronectes americanus) and their relationship with yolk proteins
- Creators
- James J NaglerDavid R Idler
- Publication Details
- Biochemistry and cell biology, Vol.68(1), pp.330-335
- Academic Unit
- UNKNOWN
- Identifiers
- 99900547184501842
- Language
- English
- Resource Type
- Journal article