Journal article
Ovine herpesvirus 2 infection in American bison: virus and host dynamics in the development of sheep-associated malignant catarrhal fever
Veterinary microbiology, Vol.159(3-4), pp.307-319
10/12/2012
Handle:
https://hdl.handle.net/2376/108273
PMID: 22592216
Abstract
Ovine herpesvirus 2 (OvHV-2) is a gammaherpesvirus that causes sheep-associated malignant catarrhal fever (SA-MCF), a frequently fatal disease mainly of ruminants. This study was designed to define virus-host dynamics following experimental OvHV-2 infection in bison. A transient peak in viral DNA accompanied by the presence of OvHV-2 ORF25, ORF50 and ORF73 transcripts was observed in lungs only from 9 to 12 days post-inoculation (DPI), suggesting occurrence of viral replication. This initial viral replication was associated with only a subtle increase in transcription of inflammation related genes in lungs and tracheal bronchial lymph nodes, while the level of expression of the majority of immune genes measured remained comparable to uninfected animals. Increasing viral load was observed in the blood and peripheral tissues at 16 and 21 DPI, respectively, indicating systemic viral dissemination. Clinical signs of MCF were observed between 28 and 35 DPI and the severity of lesions increased as disease progressed. Lesion scores were positively correlated with expression levels of ORF25, suggesting a contribution of viral replication in the pathogenesis of SA-MCF. Viral transcripts were observed in all tissues examined from 23 DPI to the end of the experiment at 35 DPI and expression levels of ORF25 were significantly higher in clinically infected animals as compared to pre-clinical stage. The data from this study provide a predictable viral-host interaction time course to test hypotheses concerning disease pathogenesis as well as mitigation of SA-MCF in susceptible species.
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Details
- Title
- Ovine herpesvirus 2 infection in American bison: virus and host dynamics in the development of sheep-associated malignant catarrhal fever
- Creators
- Cristina W Cunha - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United StatesKatherine L Gailbreath - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United StatesDonal O’Toole - Wyoming State Veterinary Laboratory, University of Wyoming, Laramie, WY, 82070-6752, United StatesDonald P Knowles - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United StatesDavid A Schneider - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United StatesStephen N White - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United StatesNaomi S Taus - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United StatesChristopher J Davies - Department of Animal, Dairy and Veterinary Sciences and Center for Integrated Biosystems, Utah State University, Logan, UT, 84322-4700, United StatesWilliam C Davis - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, United StatesHong Li - Animal Disease Research Unit, USDA-Agricultural Research Service, Washington State University, Pullman, WA, 99164-6630, United States
- Publication Details
- Veterinary microbiology, Vol.159(3-4), pp.307-319
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547070201842
- Language
- English
- Resource Type
- Journal article