Oxidative stress and senescence-like status of pear calli co-cultured on suspensions of incompatible quince microcalli

Fabio F Nocito; Luca Espen; Chiara Fedeli; Clarissa Lancilli; Stefano Musacchi; Sara Serra; Silviero Sansavini; Maurizio Cocucci; Gian Attilio Sacchi

doi:10.1093/treephys/tpq006

Back

Oxidative stress and senescence-like status of pear calli co-cultured on suspensions of incompatible quince microcalli

Journal article

Open access

Peer reviewed

Oxidative stress and senescence-like status of pear calli co-cultured on suspensions of incompatible quince microcalli

Fabio F Nocito, Luca Espen, Chiara Fedeli, Clarissa Lancilli, Stefano Musacchi, Sara Serra, Silviero Sansavini, Maurizio Cocucci and Gian Attilio Sacchi

Tree physiology, Vol.30(4), pp.450-458

04/2010

DOI: https://doi.org/10.1093/treephys/tpq006

Handle:

https://hdl.handle.net/2376/108610

PMID: 20190345

Files and links (1)

url

https://doi.org/10.1093/treephys/tpq006View

Published (Version of record) Open

Abstract

Cell Proliferation

Reactive Oxygen Species - metabolism

Receptor Cross-Talk

Oxidative Stress

Cellular Senescence

Coculture Techniques

Pyrus - metabolism

Oxygen Consumption

Transplants

Rosaceae - metabolism

This work presents a simple in vitro system to study physiological, biochemical and molecular changes occurring in a pear callus (Pyrus communis L., cv. Beurré Bosc) grown in close proximity to spatially separated undifferentiated homologous (pear) or heterologous (quince; Cydonia oblonga Mill., East Malling clone C) cells in its neighboring environment. After a 7-day co-culture period, the presence of heterologous cells produced negative effects on the pear callus, whose relative weight increase and adenylate energy charge decreased by 30 and 24%, respectively. Such behavior was associated with a higher O(2) consumption rate (+125%) which did not seem to be coupled to adenosine triphosphate synthesis. Analyses of alternative oxidase and enzymatic activities involved in reactive oxygen species (ROS) detoxification strongly suggested that the higher O(2) consumption rate, measured in the pear callus grown in the heterologous combination, may probably be ascribed to extra-respiratory activities. These, in turn, might contribute to generate metabolic scenarios where ROS-induced oxidative stresses may have the upper hand. The increase in the levels of 2-thiobarbituric acid reactive metabolites, considered as diagnostic indicators of ROS-induced lipid peroxidation, seemed to confirm this hypothesis. Moreover, reverse transcription polymerase chain reaction analysis revealed that the expression levels of a few senescence-associated genes were higher in the pear callus grown in the heterologous combination than in the homologous one. Taken as a whole, physiological and molecular data strongly suggest that undifferentiated cells belonging to a pear graft-incompatible quince clone may induce an early senescence-like status in a closely co-cultured pear callus.

Metrics

3 Record Views

Details

Title: Oxidative stress and senescence-like status of pear calli co-cultured on suspensions of incompatible quince microcalli
Creators: Fabio F Nocito - Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy
Luca Espen
Chiara Fedeli
Clarissa Lancilli
Stefano Musacchi
Sara Serra
Silviero Sansavini
Maurizio Cocucci
Gian Attilio Sacchi
Publication Details: Tree physiology, Vol.30(4), pp.450-458
Academic Unit: Horticulture, Department of
Publisher: Canada
Identifiers: 99900547049401842
Language: English
Resource Type: Journal article