Journal article
PGRMC1/2 promotes luteal vascularization and maintains the primordial follicles of mice
Reproduction (Cambridge, England), Vol.156(4), pp.365-373
10/2018
Handle:
https://hdl.handle.net/2376/101379
PMID: 30054330
Abstract
To determine whether conditional depletion of progesterone receptor membrane component (PGRMC) 1 and PGRMC2 affected ovarian follicle development, follicle distribution was assessed in ovaries of young (≈3-month-old) and middle-aged (≈6-month-old) control (Pgrmc1/2 fl/fl ) and double conditional PGRMC1/2-knockout (Pgrmc1/2 d/d ) mice. This study revealed that the distribution of primary, preantral and antral follicles was not altered in Pgrmc1/2 d/d mice, regardless of the age. Although the number of primordial follicles was similar at ≈3 months of age, their numbers were reduced by ≈80% in 6-month-old Pgrmc1/2 d/d mice compared to age-matched Pgrmc1/2 fl/fl mice. The Pgrmc1/2 d/d mice were generated using Pgr-cre mice, so ablation of Pgrmc1 and Pgrmc2 in the ovary was restricted to peri-ovulatory follicles and subsequent corpora lutea (CL). In addition, the vascularization of CL was attenuated in Pgrmc1/2 d/d mice, although mRNA levels of vascular endothelial growth factor A (Vegfa) were elevated. Moreover, depletion of Pgrmc1 and Pgrmc2 altered the gene expression profile in the non-luteal component of the ovary such that Vegfa expression, a stimulator of primordial follicle growth, was elevated; Kit Ligand expression, another stimulator of primordial follicle growth, was suppressed and anti-Mullerian hormone, an inhibitor of primordial follicle growth, was enhanced compared to Pgrmc1/2 fl/fl mice. These data reveal that luteal cell depletion of Pgrmc1 and 2 alters the expression of growth factors within the non-luteal component of the ovary, which could account for the premature demise of the adult population of primordial follicles. In , the survival of adult primordial follicles is dependent in part on progesterone receptor membrane component 1 and 2.
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Details
- Title
- PGRMC1/2 promotes luteal vascularization and maintains the primordial follicles of mice
- Creators
- John J Peluso - Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut, USAXiufang Liu - Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut, USATracy Uliasz - Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut, USACindy A Pru - Department of Animal Sciences, Center for Reproductive Biology, Washington State University, Pullman, Washington, USANicole C Kelp - Department of Animal Sciences, Center for Reproductive Biology, Washington State University, Pullman, Washington, USAJames K Pru - Department of Animal Sciences, Center for Reproductive Biology, Washington State University, Pullman, Washington, USA
- Publication Details
- Reproduction (Cambridge, England), Vol.156(4), pp.365-373
- Academic Unit
- Animal Sciences, Department of
- Publisher
- Bioscientifica Ltd
- Identifiers
- 99900546661601842
- Language
- English
- Resource Type
- Journal article