Journal article
Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis
Infection and immunity, Vol.59(6), pp.2058-2062
06/1991
Handle:
https://hdl.handle.net/2376/102556
PMCID: PMC257965
PMID: 2037365
Abstract
Mice that recovered from Ehrlichia risticii infection were immune to a challenge dose of 100 50% lethal doses. Immune or normal mouse serum was passively transferred to mice challenged with E. risticii. Clinical signs of ehrlichiosis were completely prevented in 22 of 24 recipients of immune serum, and the onset of signs of illness was delayed in the remaining two mice compared with the onset of illness in 24 of 24 recipients of nonimmune serum. Purified immunoglobulin G (IgG) was used to passively protect mice from infection with E. risticii. All 15 mice that received IgG from normal serum but none of the 15 mice that received IgG from immune serum developed clinical signs of illness. Antibodies in immune mouse serum immunoprecipitated [35S]methionine metabolically labeled E. risticii proteins with apparent molecular masses ranging from 14 to 90 kDa. The major antigens recognized by dilute immune serum in immunoblot analysis had molecular masses of 62, 53, 40, 33, 27, and 25 kDa, and the 62- and 27-kDa antigens were prominent in immunoprecipitations with dilute antibody. Antigens with molecular masses of 62, 53, 40, 33, and 27 kDa are likely surface exposed, as determined by immunoprecipitation of 125I-labeled organisms with immune mouse serum.
Metrics
6 Record Views
Details
- Title
- Passive transfer of antibody to Ehrlichia risticii protects mice from ehrlichiosis
- Creators
- P S Kaylor - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164T B Crawford - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164T F McElwain - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164G H Palmer - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164
- Publication Details
- Infection and immunity, Vol.59(6), pp.2058-2062
- Academic Unit
- Paul G. Allen School for Global Animal Health
- Identifiers
- 99900546789901842
- Language
- English
- Resource Type
- Journal article