Patterns of recombination activity on mouse chromosome 11 revealed by high resolution mapping

Timothy Billings; Evelyn E Sargent; Jin P Szatkiewicz; Nicole Leahy; Il-Youp Kwak; Nazira Bektassova; Michael Walker; Terry Hassold; Joel H Graber; Karl W Broman; Petko M Petkov

doi:10.1371/journal.pone.0015340

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Patterns of recombination activity on mouse chromosome 11 revealed by high resolution mapping

Journal article

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Patterns of recombination activity on mouse chromosome 11 revealed by high resolution mapping

Timothy Billings, Evelyn E Sargent, Jin P Szatkiewicz, Nicole Leahy, Il-Youp Kwak, Nazira Bektassova, Michael Walker, Terry Hassold, Joel H Graber, Karl W Broman, …

PloS one, Vol.5(12), pp.e15340-e15340

12/08/2010

DOI: https://doi.org/10.1371/journal.pone.0015340

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https://hdl.handle.net/2376/105905

PMCID: PMC2999565

PMID: 21170346

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Abstract

Crossing Over, Genetic

Mice, Inbred C57BL

Genotype

Male

Chromosome Mapping

Meiosis

Animals

Recombination, Genetic

Alleles

Female

Mice

Models, Genetic

Genome

Crosses, Genetic

The success of high resolution genetic mapping of disease predisposition and quantitative trait loci in humans and experimental animals depends on the positions of key crossover events around the gene of interest. In mammals, the majority of recombination occurs at highly delimited 1-2 kb long sites known as recombination hotspots, whose locations and activities are distributed unevenly along the chromosomes and are tightly regulated in a sex specific manner. The factors determining the location of hotspots started to emerge with the finding of PRDM9 as a major hotspot regulator in mammals, however, additional factors modulating hotspot activity and sex specificity are yet to be defined. To address this limitation, we have collected and mapped the locations of 4829 crossover events occurring on mouse chromosome 11 in 5858 meioses of male and female reciprocal F1 hybrids of C57BL/6J and CAST/EiJ mice. This chromosome was chosen for its medium size and high gene density and provided a comparison with our previous analysis of recombination on the longest mouse chromosome 1. Crossovers were mapped to an average resolution of 127 kb, and thirteen hotspots were mapped to <8 kb. Most crossovers occurred in a small number of the most active hotspots. Females had higher recombination rate than males as a consequence of differences in crossover interference and regional variation of sex specific rates along the chromosome. Comparison with chromosome 1 showed that recombination events tend to be positioned in similar fashion along the centromere-telomere axis but independently of the local gene density. It appears that mammalian recombination is regulated on at least three levels, chromosome-wide, regional, and at individual hotspots, and these regulation levels are influenced by sex and genetic background but not by gene content.

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Title: Patterns of recombination activity on mouse chromosome 11 revealed by high resolution mapping
Creators: Timothy Billings - Center for Genome Dynamics, The Jackson Laboratory, Bar Harbor, Maine, United States of America
Evelyn E Sargent
Jin P Szatkiewicz
Nicole Leahy
Il-Youp Kwak
Nazira Bektassova
Michael Walker
Terry Hassold
Joel H Graber
Karl W Broman
Petko M Petkov
Publication Details: PloS one, Vol.5(12), pp.e15340-e15340
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: R01 GM078452 / NIGMS NIH HHS P50GM076468 / NIGMS NIH HHS P30 CA034196 / NCI NIH HHS R01HD21341 / NICHD NIH HHS P50 GM076468 / NIGMS NIH HHS R01GM078452 / NIGMS NIH HHS T32 HD007065 / NICHD NIH HHS R01 HD021341 / NICHD NIH HHS R37 HD021341 / NICHD NIH HHS CA34196 / NCI NIH HHS
Identifiers: 99900546785301842
Language: English
Resource Type: Journal article