Journal article
Pd@Pt nanoparticle-linked immunosorbent assay for quantification of Collagen type II
Analytica chimica acta, Vol.1266, pp.341265-341265
07/25/2023
PMID: 37244654
Abstract
The evaluation of specific protein content in engineered tissues provides a gateway for developing regenerative medicine treatments. Since collagen type II, the major component of articular cartilage, is critical for the blossoming field of articular cartilage tissue engineering, the interest in this protein is growing rapidly. Accordingly, the need for quantification of collagen type II is increasing as well. In this study, we provide recent results for a new quantifying nanoparticle sandwich immunoassay technique for collagen type II. Since mesoporous palladium@platinum (Pd@Pt) nanoparticles have peroxidase-like catalytic activities, these nanoparticles were utilized in an enzyme-linked immunosorbent assay (ELISA)-like format to circumvent the need for traditional enzymes. These nanoparticles were easily conjugated with anti-collagen type II antibodies by the natural affinity interaction and used to develop a direct sandwich ELISA-like format for nanoparticle-linked immunosorbent assays. Using this method, we obtained a limit of detection of 1 ng mL−1, a limit of quantification of 9 ng mL-1. and a broad linear range between 1 ng mL−1 and 50 μg mL−1 for collagen type II with an average relative standard deviation of 5.5%, useable over a pH range of 7 – 9 at least. The assay was successfully applied to quantify collagen type II in cartilage tissues and compared with the results of commercial ELISAs and gene expression by reverse transcription-quantitative polymerase chain reaction. This method provides a thermally stable and cost-efficient alternative to traditional ELISAs. It also extends the application of nanoparticle-linked immunosorbent assays, thereby providing the potential to quantify other proteins and apply the technology in the medical, environmental, and biotechnology industry fields.
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•Pd@Pt nanoparticle-linked immunosorbent assay for collagen type II quantification.•A direct sandwich ELISA-like format with Pd@Pt nanoparticles.•An LOD of 1 ng mL−1 with a linear range of 1 ng mL−1 to 50 μg mL−1
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Details
- Title
- Pd@Pt nanoparticle-linked immunosorbent assay for quantification of Collagen type II
- Creators
- Eunice Y. Kwon - Washington State UniversityHaneen A. Abusharkh - Washington State UniversityXiaofan Ruan - Washington State UniversityDan Du - Washington State UniversityEllis Hammond-Pereira - Washington State UniversityBernard J. Van Wie - Washington State University
- Publication Details
- Analytica chimica acta, Vol.1266, pp.341265-341265
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier B.V
- Identifiers
- 99901227838301842
- Language
- English
- Resource Type
- Journal article