Journal article
Phenotype matters: identification of light-responsive cells in the mouse suprachiasmatic nucleus
The Journal of neuroscience, Vol.24(1), pp.68-75
01/07/2004
Handle:
https://hdl.handle.net/2376/104123
PMCID: PMC3271855
PMID: 14715939
Abstract
The suprachiasmatic nucleus (SCN) of the hypothalamus is the neural locus of the circadian clock. To explore the organization of the SCN, two strains of transgenic mice, each bearing a jellyfish green fluorescent protein (GFP) reporter, were used. In one, GFP was driven by the promoter region of the mouse Period1 gene (mPer1) (Per1::GFP mouse), whereas in the other, GFP was inserted in the promoter region of calbindin-D(28K)-bacterial artificial chromosome (CalB::GFP mouse). In the latter mouse, GFP-containing SCN cells are immunopositive for gastrin-releasing peptide. In both mouse lines, light-induced Per1 mRNA and Fos are localized to the SCN subregion containing gastrin-releasing peptide. Double-label immunohistochemistry reveals that most gastrin-releasing peptide cells (approximately 70%) contain Fos after a brief light pulse. To determine the properties of SCN cells in this light-responsive region, we examined the expression of rhythmic Period genes and proteins. Gastrin-releasing peptide-containing cells do not express detectable rhythms in these key components of the molecular circadian clock. The results support the view that the mammalian SCN is composed of functionally distinct cell groups, of which some are light induced and others are rhythmic with respect to clock gene expression. Furthermore, the findings suggest that gastrin-releasing peptide is a potential mediator of intercellular communication between light-induced and oscillator cells within the SCN.
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Details
- Title
- Phenotype matters: identification of light-responsive cells in the mouse suprachiasmatic nucleus
- Creators
- Ilia N Karatsoreos - Department of Psychology, Columbia University, New York, New York 10027, USALily YanJoseph LeSauterRae Silver
- Publication Details
- The Journal of neuroscience, Vol.24(1), pp.68-75
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- United States
- Grant note
- R01 NS037919-04 / NINDS NIH HHS 37919 / PHS HHS R01 MH063341-01 / NIMH NIH HHS R01 NS037919 / NINDS NIH HHS R01 MH063341 / NIMH NIH HHS 63341 / PHS HHS
- Identifiers
- 99900547097101842
- Language
- English
- Resource Type
- Journal article