Journal article
Phorbol ester has different effects on forskolin and β-adrenergic-stimulated cAMP accumulation in mouse parotid acini
Cellular signalling, Vol.5(5), pp.583-592
1993
Handle:
https://hdl.handle.net/2376/117889
PMID: 7508731
Abstract
Phorbol 12-myristate 13-acetate (TPA) augmented the effects of forskolin, and inhibited the effects of isoproterenol on cAMP accumulation in mouse parotid acini. Treatment of intact cells with the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine (MIX), blocked TPA inhibition of isoproterenol but not forskolin-stimulated cAMP accumulation. TPA also caused the translocation of protein kinase C (PKC) from cytosol to membrane. Pre-treatment of parotid acini with TPA for 30 min enhanced the forskolin and isoproterenol-stimulated adenylate cyclase activity in isolated parotid membranes. Addition of purified PKC (nPKC) to parotid membranes mimicked the effects of TPA in increasing cAMP synthesis; the effects were blocked in the absence of calcium and phospholipid, and in the presence of the systhetic peptide (PKC 19–36). Purified PKC also mimicked the effects of TPA in augmenting forskolin and isoproterenol-stimulated adenylate cyclase activities in the cell-free system. Data suggest that the differential regulation of forskolin and isoproterenol-stimulated cAMP accumulation by TPA results from modification of enzymes that synthesize and degrade cAMP.
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Details
- Title
- Phorbol ester has different effects on forskolin and β-adrenergic-stimulated cAMP accumulation in mouse parotid acini
- Creators
- Eileen L Watson - Departments of Oral Biology, University of Washington, Seattle, WA 98195, U.S.AKerry Jacobson - Departments of Oral Biology, University of Washington, Seattle, WA 98195, U.S.AKathryn Meier - Department of Pharmacology, University of Washington, Seattle, WA 98195, U.S.A
- Publication Details
- Cellular signalling, Vol.5(5), pp.583-592
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900548459301842
- Language
- English
- Resource Type
- Journal article