Journal article
Phosphorylation of CBP Mediates Transcriptional Activation by Neural Activity and CaM Kinase IV
Neuron (Cambridge, Mass.), Vol.34(2), pp.235-244
2002
Handle:
https://hdl.handle.net/2376/100335
PMID: 11970865
Abstract
Activity-regulated transcription has been implicated in adaptive plasticity in the CNS. In many instances, this plasticity depends upon the transcription factor CREB. Precisely how neuronal activity regulates CREB remains unclear. To address this issue, we examined the phosphorylation state of components of the CREB transcriptional pathway. We show that NMDA activates transcription of CREB-responsive genes in hippocampal neurons, with ERK responsible for persistent CREB phosphorylation and CaM kinase IV (CaMKIV) responsible for phosphorylating the CREB coactivator, CBP. Ser301 of CBP was identified as a major target of CaMKIV phosphorylation in vitro and in vivo. CaM kinase inhibitors attenuated phosphorylation at Ser301 and blocked CBP-dependent transcription. Additionally, mutation of Ser301 impaired NMDA- and CaMKIV-stimulated transcription. These findings demonstrate that activity-induced CaMKIV signaling contributes to CREB/CBP-dependent transcription by phosphorylating CBP at Ser301.
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Details
- Title
- Phosphorylation of CBP Mediates Transcriptional Activation by Neural Activity and CaM Kinase IV
- Creators
- Soren Impey - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USAAmy L Fong - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USAYanhong Wang - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USAJean-Rene Cardinaux - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USADaniel M Fass - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USAKarl Obrietan - Department of Neuroscience, Ohio State University, 4018 Graves Hall, 333 W. 10th Avenue, Columbus, OH 43210 USAGary A Wayman - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USADaniel R Storm - Department of Pharmacology, University of Washington, School of Medicine, J-681-D Health Sciences Center, Box 357750, Seattle, WA 98195 USAThomas R Soderling - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USARichard H Goodman - The Vollum Institute, Oregon Health Sciences University, 3181 SW Sam Jackson Park Road, L-474, Portland, OR 97201 USA
- Publication Details
- Neuron (Cambridge, Mass.), Vol.34(2), pp.235-244
- Academic Unit
- Integrative Physiology and Neuroscience, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900546528501842
- Language
- English
- Resource Type
- Journal article