Journal article
Photoswitching-induced frequency-locked donor-acceptor fluorescence double modulations identify the target analyte in complex environments
Journal of the American Chemical Society, Vol.133(40), pp.16092-16100
10/12/2011
Handle:
https://hdl.handle.net/2376/116200
PMID: 21863862
Abstract
Precisely identifying biological targets and accurately extracting their relatively weak signals from complicated physiological environments represent daunting challenges in biological detection and biomedical diagnosis. Fluorescence techniques have become the method of choice and offer minimally invasive and ultrasensitive detections, thus, providing a wealth of information regarding the biological mechanisms in living systems. Despite fluorescence analysis has advanced remarkably, conventional detections still encounter considerable limitations. This stems from the fact that the fluorescence intensity signal (I) is sensitive and liable to numerous external factors including temperature, light source, medium characteristics, and dye concentration. The interferences exasperatingly undermine the precision of measurements, and frequently render the signal undetectable. For example, fluorescence from single-molecule emitters can be measured on glass substrates under optimum conditions, but single-molecule events in complicated physiological environments such as live cells can hardly be detected because of autofluorescence interference and other factors. Furthermore, traditional intensity (I) and wavelength (λ) measurements do not reveal the interactive nature between the donor and the acceptor. Thus, innovative detection strategies to circumvent these aforementioned limitations of the conventional techniques are critically needed. With the use of photoswitching-induced donor-acceptor-fluorescence double modulations, we present a novel strategy that introduces three additional physical parameters: modulation amplitude (A), phase shift (ΔΦ), and lock-in frequency (ω), and demonstrate that such a strategy can circumvent the limitation of the conventional fluorescence detection techniques. Together, these five physical quantities (I, λ, A, ΔΦ, ω) reveal insightful information regarding molecular interactive strength between the probe and the analyte and enable extracting weak-fluorescence spectra from large interfering noises in complex environments.
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Details
- Title
- Photoswitching-induced frequency-locked donor-acceptor fluorescence double modulations identify the target analyte in complex environments
- Creators
- Zhiyuan Tian - College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing, PR China 100049. zytian@gucas.ac.cnWuwei WuWei WanAlexander D Q Li
- Publication Details
- Journal of the American Chemical Society, Vol.133(40), pp.16092-16100
- Academic Unit
- Department of Chemistry
- Publisher
- United States
- Identifiers
- 99900547995201842
- Language
- English
- Resource Type
- Journal article