Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families

Buel D Rodgers; Eric H Roalson; Cullen Thompson

doi:10.1016/j.ygcen.2007.04.013

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Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families

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Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families

Buel D Rodgers, Eric H Roalson and Cullen Thompson

General and comparative endocrinology, Vol.155(1), pp.201-207

2008

DOI: https://doi.org/10.1016/j.ygcen.2007.04.013

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https://hdl.handle.net/2376/110662

PMCID: PMC2241922

PMID: 17537438

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Abstract

IGFBP

Phylogenetics

IGFBP-rP

Insulin-like growth factor (IGF) activity is regulated by six high affinity binding proteins (IGFBPs) and possibly by some of the nine IGFBP-related proteins (IGFBP-rPs). To determine the phylogenetic relationship of this proposed gene superfamily, we conducted maximum likelihood (ML) and Bayesian inference analyses on a matrix of amino acid sequences from a diversity of vertebrate species. A single most likely phylogram, ML bootstrap, and Bayesian consensus tree of 10,000,000 generations revealed a monophyletic IGFBP lineage independent of the IGFBP-rPs. The IGFBPs segregated into three distinct clades: IGFBP-1, -3, and -6. Subsequent gene duplication events within the IGFBP-1 and -3 clades resulted in the production and divergence of IGFBP-2 and -4 within the IGFBP-1 clade and IGFBP-5 in the IGFBP-3 clade. By contrast, the IGFBP-rPs were distributed paraphyletically into two clades: IGFBP-rP1, 5, and 6 in one clade and the CCN family (IGFBP-rP2–4,7–9) in another. A recently identified IGFBP-3 homolog in rainbow trout localized to the IGFBP-2 subclade. Subsequence analysis identified a RGD motif common to IGFBP-2 orthologs, but did not identify the nuclear localization sequence present in IGFBP-3 and -5 homologs. The putative trout IGFBP-3 was 36–55% identical to different IGFBP-2 proteins, but only 24–27% identical to IGFBP-3 proteins. These results suggest that the IGFBPs and IGFBP-rPs are at best distantly related and that the limited similarities likely resulted from exon shuffling. They also suggest that rainbow trout, and possibly other salmonids, possess two IGFBP-2 paralogs as the putative trout IGFBP-3 is misannotated.

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Title: Phylogenetic analysis of the insulin-like growth factor binding protein (IGFBP) and IGFBP-related protein gene families
Creators: Buel D Rodgers - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA
Eric H Roalson - School of Biological Sciences, Washington State University, Pullman, WA 99164-6351, USA
Cullen Thompson - Department of Animal Sciences, Washington State University, Pullman, WA 99164-6351, USA
Publication Details: General and comparative endocrinology, Vol.155(1), pp.201-207
Academic Unit: Biological Sciences, School of
Publisher: Elsevier Inc
Identifiers: 99900547236901842
Language: English
Resource Type: Journal article