Physical mapping of inhibin beta-A in domestic cattle

H L Neibergs; D S Gallagher; M Georges; L S Sargeant; A B Dietz; J E Womack

doi:10.1007/BF00357092

Back

Physical mapping of inhibin beta-A in domestic cattle

Journal article

Peer reviewed

Physical mapping of inhibin beta-A in domestic cattle

H L Neibergs, D S Gallagher, M Georges, L S Sargeant, A B Dietz and J E Womack

Mammalian genome, Vol.4(6), pp.328-332

1993

DOI: https://doi.org/10.1007/BF00357092

Handle:

https://hdl.handle.net/2376/107698

PMID: 8100459

Abstract

Cricetinae

Hybrid Cells

Molecular Sequence Data

In Situ Hybridization, Fluorescence

Chromosome Mapping

Cattle - genetics

Polymorphism, Genetic

Animals

Polymorphism, Restriction Fragment Length

Base Sequence

Polymerase Chain Reaction

Inhibins - genetics

DNA, Single-Stranded

CHO Cells

The gene for the beta-A subunit of inhibin (INHBA) was assigned to bovine syntenic group U13 by bovine x rodent hybrid somatic cells and the polymerase chain reaction (PCR). A 482-bp PCR fragment was used to clone a 37-kb cosmid. This cosmid was assigned to bovine Chromosome (Chr) 4 (BTA 4) by fluorescence in situ hybridization (FISH). This is the first assignment of a U13 marker to a bovine chromosome. A restriction fragment length polymorphism (RFLP) was detected with PstI within the INHBA cosmid.

Metrics

15 Record Views

Details

Title: Physical mapping of inhibin beta-A in domestic cattle
Creators: H L Neibergs - Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843
D S Gallagher
M Georges
L S Sargeant
A B Dietz
J E Womack
Publication Details: Mammalian genome, Vol.4(6), pp.328-332
Academic Unit: Animal Sciences, Department of
Publisher: United States
Identifiers: 99900546986901842
Language: English
Resource Type: Journal article

Physical mapping of inhibin beta-A in domestic cattle

Related links

Abstract

Metrics

Details